eIF4E Antibody, HRP Conjugated
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Catalog numberbs-3621R-HRP
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Price:
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Size0.1ml
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Long nameeIF4E Polyclonal Antibody, HRP Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationHRP
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target Antigene4E
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SpecificityThis is a highly specific antibody against e4E.
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ModificationUnmodified
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Modification siteNone
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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SourceKLH conjugated synthetic peptide derived from human eIF4E
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Gene ID number1977
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Tested applicationsWB, IHC-P
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500)
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CrossreactivityHuman, Mouse, Rat
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Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen backgroundeIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation EmissionN/A
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SynonymsE4F; e-4E; e 4E; CBP; e 4F 25 kDa subunit; CBP; e 4E; e 4F 25 kDa subunit; E 4F; E4E1; E4EL1; E4F; Eukaryotic Translation Initiation Factor 4 E; Eukaryotic translation initiation factor 4E like 1; Messanger RNA Cap Binding Protein e 4E.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Additional conjugationHRP Conjugated
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French translationanticorps
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Gene target
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Short nameAnti-e4E
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TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
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IsotypeImmunoglobulin G (IgG)
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LabelHRP
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Alternative nameAnti-e4E PAb HRP
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data