E2F1 (Ser364) Antibody, Cy3 Conjugated
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Catalog number
bs-4076R-Cy3
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Price
Please ask
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Size
0.1ml
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Long name
E2F1 (Ser364) Polyclonal Antibody, Cy3 Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Cy3
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
E2F1 Ser364
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Specificity
This is a highly specific antibody against E2F1 Ser364.
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Modification
Phosphorylation
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Modification site
Ser364
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
350-370/437
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Subcellular location
Nucleus
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Source
KLH conjugated synthetic phosphopeptide derived from human E2F1 around the phosphorylation site of Ser364
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Gene ID number
1869
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Swiss Prot
Q01094
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
512,550nm/570,615nm
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Synonyms
RBP3; E2F-1; RBAP1; RBBP3; Transcription factor E2F1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1; E2F1
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-E2F1 Ser364 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
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Additional conjugation
cy3 conjugation kit
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French translation
anticorps
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Gene target
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Gene symbol
E2F1
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Short name
Anti-E2F1 Ser364
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Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
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Isotype
Immunoglobulin G (IgG)
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Label
Cy3
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Alternative name
Anti-E2F1 Ser364 PAb Cy3
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Alternative technique
antibodies
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Alternative to gene target
E2F transcription factor 1, E2F-1 and RBAP1 and RBBP3 and RBP3, E2F1 and IDBG-67214 and ENSG00000101412 and 1869, sequence-specific DNA binding, nuclei, E2f1 and IDBG-210416 and ENSMUSG00000027490 and 13555, BT.78028 and IDBG-641855 and ENSBTAG00000003971 and 535369
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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