DNA Ligase IV/LIG4 Antibody, HRP Conjugated
-
Catalog number
bs-1677R-HRP
-
Price
Please ask
-
Size
0.1ml
-
-
Long name
DNA Ligase IV/LIG4 Polyclonal Antibody, HRP Conjugated
-
Category
Conjugated Primary Antibodies
-
Conjugation
HRP
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
DNA Ligase IV/LIG4
-
Specificity
This is a highly specific antibody against DNA Ligase IV/LIG4.
-
Modification
Unmodified
-
Modification site
None
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Immunogen_range
60-110/911
-
Subcellular location
Nucleus
-
Source
KLH conjugated synthetic peptide derived from human DNA Ligase IV
-
Gene ID number
3981
-
Swiss Prot
P49917
-
Tested applications
WB, IHC-P
-
Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500)
-
Crossreactivity
Human, Mouse, Rat
-
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Antigen background
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
-
Purification method
This antibody was purified via Protein A.
-
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
-
Excitation Emission
N/A
-
Synonyms
LIG4S; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4
-
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
-
Additional conjugation
HRP Conjugated
-
French translation
anticorps
-
Gene target
-
Gene symbol
LIG4, PRKDC, HLA-DOA
-
Short name
Anti-DNA Ligase IV/LIG4
-
Technique
Antibody, dna, antibodies against human proteins, antibodies for, antibody Conjugates
-
Isotype
Immunoglobulin G (IgG)
-
Label
HRP
-
Alternative name
Anti-DNA Ligase IV/LIG4 PAb HRP
-
Alternative technique
antibodies
-
Alternative to gene target
ligase IV, DNA, ATP-dependent, LIG4S, LIG4 and IDBG-49744 and ENSG00000174405 and 3981, metal ion binding, nuclei, Lig4 and IDBG-131943 and ENSMUSG00000049717 and 319583, LIG4 and IDBG-633610 and ENSBTAG00000015868 and 781252
-
Gene info
-
Identity
-
Gene
-
Long gene name
DNA ligase 4
-
Synonyms gene name
- ligase IV, DNA, ATP-dependent
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1995-08-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
-
Locus Specific Databases
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
-
Tree numbers
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
