DNA Ligase IV/LIG4 Antibody, Cy3 Conjugated
-
Catalog number
bs-1677R-Cy3
-
Price
Please ask
-
Size
0.1ml
-
-
Long name
DNA Ligase IV/LIG4 Polyclonal Antibody, Cy3 Conjugated
-
Category
Conjugated Primary Antibodies
-
Conjugation
Cy3
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
DNA Ligase IV/LIG4
-
Specificity
This is a highly specific antibody against DNA Ligase IV/LIG4.
-
Modification
Unmodified
-
Modification site
None
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Immunogen_range
60-110/911
-
Subcellular location
Nucleus
-
Source
KLH conjugated synthetic peptide derived from human DNA Ligase IV
-
Gene ID number
3981
-
Swiss Prot
P49917
-
Tested applications
IF(IHC-P)
-
Recommended dilutions
IF(IHC-P)(1:50-200)
-
Crossreactivity
Human, Mouse, Rat
-
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Antigen background
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
-
Purification method
This antibody was purified via Protein A.
-
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
-
Excitation Emission
512,550nm/570,615nm
-
Synonyms
LIG4S; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4
-
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-DNA Ligase IV/LIG4 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
-
Additional conjugation
cy3 conjugation kit
-
French translation
anticorps
-
Gene target
-
Gene symbol
LIG4, PRKDC, HLA-DOA
-
Short name
Anti-DNA Ligase IV/LIG4
-
Technique
Antibody, dna, antibodies against human proteins, antibodies for, antibody Conjugates
-
Isotype
Immunoglobulin G (IgG)
-
Label
Cy3
-
Alternative name
Anti-DNA Ligase IV/LIG4 PAb Cy3
-
Alternative technique
antibodies
-
Alternative to gene target
ligase IV, DNA, ATP-dependent, LIG4S, LIG4 and IDBG-49744 and ENSG00000174405 and 3981, metal ion binding, nuclei, Lig4 and IDBG-131943 and ENSMUSG00000049717 and 319583, LIG4 and IDBG-633610 and ENSBTAG00000015868 and 781252
-
Gene info
-
Identity
-
Gene
-
Long gene name
DNA ligase 4
-
Synonyms gene name
- ligase IV, DNA, ATP-dependent
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1995-08-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
-
Locus Specific Databases
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
