CPA3/MC-CPA Antibody, FITC Conjugated

• Catalog number
  bs-12338R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  CPA3/MC-CPA Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  CPA3/MC-CPA
• Specificity
  This is a highly specific antibody against CPA3/MC-CPA.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic peptide derived from human CPA3/MC-CPA
• Gene ID number
  1359
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Carboxypeptidase A (CPA) is a pancreatic exopeptidase which hydrolyses the peptide bond adjacent to the C-terminal end in polypeptide chains. Mast cell carboxypeptidase A (MC-CPA), a part of the peptidase M14 family, is a highly conserved metalloprotease localized to the secretory granules, along with trytases and chymases. MC-CPA is stored as an active enzyme in the granule and is released, along with other inflammatory mediators, upon mast cell degranulation. MC-CPA mirrors pancreatic carboxypeptidase A in cleaving COOH-terminal aromatic and aliphatic amino acid residues. The optimum pH of MC-CPA is between neutral and basic, depending upon the substrate. The MC-CPA gene, CPA3, resides on chromosome 3 and contains 11 exons.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  carboxypeptidase A; Carboxypeptidase A3; carboxypeptidase A3 mast cell; CBPA3_HUMAN; Cpa3; Mast cell carboxypeptidase A; mast cell carboxypeptidase A3; MC CPA; MC-CPA.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  CPA3/MC-CPA   Conjugated  
• Gene symbol
  CPA3, CPA1, CPA4
• Short name
  Anti-CPA3/MC-CPA
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-CPA3/MC-CPA PAb FITC
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  carboxypeptidase A3 (mast cell), MC-CPA, CPA3 and IDBG-60396 and ENSG00000163751 and 1359, zinc ion binding, Extracellular, Cpa3 and IDBG-133537 and ENSMUSG00000001865 and 12873, CPA3 and IDBG-637892 and ENSBTAG00000015008 and 528454

Gene info

• Identity
  HGNC:2298
• Gene
  CPA3
• Long gene name
  carboxypeptidase A3
• Synonyms gene name
  • carboxypeptidase A3 (mast cell)
• Synonyms name
  • mast cell carboxypeptidase A
  • tissue carboxypeptidase A
• Locus
  3q24
• Discovery year
  1990-06-13
• Entrez gene record
  1359
• RefSeq identity
  • NM_001870
• Classification
  • M14 carboxypeptidases
• VEGA ID
  OTTHUMG00000159526

Gene info

• Identity
  HGNC:2296
• Gene
  CPA1
• Long gene name
  carboxypeptidase A1
• Synonyms gene
  • CPA
• Synonyms gene name
  • carboxypeptidase A1 (pancreatic)
• Synonyms name
  • pancreatic carboxypeptidase A
• Locus
  7q32.2
• Discovery year
  1986-01-01
• Entrez gene record
  1357
• RefSeq identity
  • NM_001868
• Classification
  • M14 carboxypeptidases
• VEGA ID
  OTTHUMG00000157826

Gene info

• Identity
  HGNC:15740
• Gene
  CPA4
• Long gene name
  carboxypeptidase A4
• Synonyms
  • CPA3
• Synonyms name
  • carboxypeptidase A3
• GenBank acession
  • AF095719
• Locus
  7q32.2
• Discovery year
  2001-05-24
• Entrez gene record
  51200
• Pubmed identfication
  • 10383164
  • 10860668
• RefSeq identity
  • NM_016352
• Classification
  • M14 carboxypeptidases
• VEGA ID
  OTTHUMG00000157825
• Locus Specific Databases
  • LRG_1038

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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