Collagen II Antibody, Biotin Conjugated
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Catalog number
bs-0709R-Biotin
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Price
Please ask
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Size
0.1ml
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Long name
Collagen II Polyclonal Antibody, Biotin Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Biotin
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
Collagen II
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Specificity
This is a highly specific antibody against Collagen II.
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Modification
Unmodified
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Modification site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
1230-1280/1487
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Subcellular location
Extracellular, Secreted
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Source
KLH conjugated synthetic peptide derived from human Collagen II
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Gene ID number
1280
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Swiss Prot
P02458
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Tested applications
WB, ELISA, IHC-P
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Recommended dilutions
WB(1:100-1000), ELISA(1:500-1000), IHC-P(1:100-500)
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Crossreactivity
Human, Mouse, Rat, Rabbit
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
N/A
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Synonyms
AOM; ANFH; SEDC; STL1; COL11A3; Collagen alpha-1(II) chain; Alpha-1 type II collagen; COL2A1
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugation
Biotinylated
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Gene
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
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French translation
anticorps
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Gene target
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Short name
Anti-Collagen II
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Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
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Isotype
Immunoglobulin G (IgG)
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Label
Biotin
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Alternative name
Anti-Collagen II PAb Biotin
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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