CHEK1(Ser317) Antibody, Cy5.5 Conjugated

• Catalog number
  bs-5251R-Cy5.5
• Price
  Please ask
• Size
  0.1ml

• Long name
  CHEK1(Ser317) Polyclonal Antibody, Cy5.5 Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Cy5.5
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  CHEK1 Ser317
• Specificity
  This is a highly specific antibody against CHEK1 Ser317. This phosphorylation site is homologous to that of Ser317 in Mouse and Rat.
• Modification
  Phosphorylation
• Modification site
  Ser317
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  280-330/476
• Subcellular location
  Cytoplasm, Nucleus
• Source
  KLH conjugated synthetic phosphopeptide derived from human CHEK1 around the phosphorylation site of Ser317
• Gene ID number
  1111
• Swiss Prot
  O14757
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  675nm/694nm
• Synonyms
  CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1; CHEK1
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
• Additional conjugation
  Cy5.5, Cy5.5
• Conjugated
  These antibodies are excite for emission at 678nm and detected at a 694 nm wavelengths.
• French translation
  anticorps

• Gene target
  CHEK1   Ser317   Conjugated  
• Gene symbol
  CHEK1
• Short name
  Anti-CHEK1 Ser317
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Cy5.5
• Alternative name
  Anti-CHEK1 Ser317 PAb Cy5.5
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:1925
• Gene
  CHEK1
• Long gene name
  checkpoint kinase 1
• Synonyms gene name
  • CHK1 (checkpoint, S.pombe) homolog
  • CHK1 checkpoint homolog (S. pombe)
• Synonyms
  • CHK1
• GenBank acession
  • AF016582
  • BC017575
• Locus
  11q24.2
• Discovery year
  1998-04-21
• Entrez gene record
  1111
• Pubmed identfication
  • 9278511
  • 9382850
• RefSeq identity
  • NM_001274
• VEGA ID
  OTTHUMG00000165853

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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