CD163/M130 Antibody, FITC Conjugated

• Catalog number
  bs-2527R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  CD163/M130 Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  CD163/M130
• Specificity
  This is a highly specific antibody against CD163/M130.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  800-850/1156
• Subcellular location
  Extracellular
• Source
  KLH conjugated synthetic peptide derived from human CD163/M130
• Gene ID number
  9332
• Swiss Prot
  Q86VB7
• Tested applications
  FCM, IF(IHC-P), IF(ICC)
• Recommended dilutions
  FCM(1:20-100), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  M13; MM13; Scavenger receptor cysteine-rich type 1 protein M13; Hemoglobin scavenger receptor; CD163
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  CD163/M130   Conjugated  
• Gene symbol
  CD163, PPP1R12A
• Short name
  Anti-CD163/M130
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-CD163/M130 PAb FITC
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  CD163 molecule, M130 and MM130, CD163 and IDBG-16292 and ENSG00000177575 and 9332, protein binding, Extracellular, Cd163 and IDBG-186721 and ENSMUSG00000008845 and 93671, CD163 and IDBG-639861 and ENSBTAG00000019669 and 533844

Gene info

• Identity
  HGNC:1631
• Gene
  CD163
• Long gene name
  CD163 molecule
• Synonyms gene name
  • CD163 antigen
• Synonyms
  • M130
  • MM130
  • SCARI1
• GenBank acession
  • Z22968
• Locus
  12p13.31
• Discovery year
  2000-01-18
• Entrez gene record
  9332
• Pubmed identfication
  • 10403791
  • 8370408
• RefSeq identity
  • NM_004244
  • NM_203416
• Classification
  • CD molecules
  • Scavenger receptors
  • Scavenger receptor cysteine rich domain containing
• VEGA ID
  OTTHUMG00000168353

Gene info

• Identity
  HGNC:7618
• Gene
  PPP1R12A
• Long gene name
  protein phosphatase 1 regulatory subunit 12A
• Synonyms gene
  • MYPT1
• Synonyms gene name
  • protein phosphatase 1, regulatory (inhibitor) subunit 12A
  • protein phosphatase 1, regulatory subunit 12A
• Synonyms
  • MBS
  • M130
• Synonyms name
  • myosin phosphatase-targeting subunit 1
  • myosin binding subunit
• GenBank acession
  • D87930
• Locus
  12q21.2-q21.31
• Discovery year
  1997-12-23
• Entrez gene record
  4659
• Pubmed identfication
  • 9286714
• RefSeq identity
  • NM_002480
• Classification
  • Ankyrin repeat domain containing
  • Myosin phosphatase targeting family
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000170100

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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