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Long name
c-Met(Tyr1234 + Tyr1235) Antibody, Cy7 Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Cy7
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
c-Met Tyr1234 + Tyr1235
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Specificity
This is a highly specific antibody against c-Met Tyr1234 + Tyr1235. These phosphorylation sites are homologous to Tyr1232 + Ty1233 in Mouse and Tyr1235 + Tyr1236 in Rat. Due to the highly conserved nature of this domain, there is a chance that this antibody will react with MSP receptors in Mouse and, less likely, in Human when phosphorylated at the the sites of Ser1238 and Ser1239 in Human, Ser1215 and Ser1216 in Mouse. No phosphorylation information is currently available Rat.
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Modification
Phosphorylation
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Modification site
Tyr1234 + Tyr1235
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
1220-1240/1390
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Subcellular location
Helical
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Source
KLH conjugated synthetic phosphopeptide derived from human c-Met around the phosphorylation site of Tyr1234/1235
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Gene ID number
4233
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Swiss Prot
P08581
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
743nm/767nm
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Synonyms
HGFR; AUTS9; RCCP2; c-Met; Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met; MET
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugated
These antibodies are excite for emission at 650 nm and detected at a 676 nm wavelengths.
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Additional conjugation
Cy7
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French translation
anticorps