c-Fos (Thr325) Antibody, HRP Conjugated
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Catalog number
bs-3154R-HRP
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Price
Please ask
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Size
0.1ml
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Long name
c-Fos (Thr325) Polyclonal Antibody, HRP Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
HRP
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
c-Fos Thr325
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Specificity
This is a highly specific antibody against c-Fos Thr325. This phosphorylation site is homologous to Thr325 in Mouse and Rat.
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Modification
Phosphorylation
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Modification site
Thr325
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
300-350/380
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Subcellular location
Cytoplasm, Nucleus
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Source
KLH conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325
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Gene ID number
2353
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Swiss Prot
P01100
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Tested applications
WB, IHC-P
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Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
N/A
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Synonyms
p55; AP-1; C-FOS; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7; FOS; G0S7
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Additional conjugation
HRP Conjugated
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French translation
anticorps
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Gene target
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Short name
Anti-c-Fos Thr325
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Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
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Isotype
Immunoglobulin G (IgG)
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Label
HRP
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Alternative name
Anti-c-Fos Thr325 PAb HRP
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Alternative technique
antibodies
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Alternative to gene target
FBJ murine osteosarcoma viral oncogene homolog, AP-1 and C-FOS and p55, FOS and IDBG-12957 and ENSG00000170345 and 2353, R-SMAD binding, nuclei, Fos and IDBG-158538 and ENSMUSG00000021250 and 14281, FOS and IDBG-646869 and ENSBTAG00000004322 and 280795
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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