Bid (Ser61) Antibody, Cy3 Conjugated
-
Catalog number
bs-5231R-Cy3
-
Price
Please ask
-
Size
0.1ml
-
-
Long name
Bid (Ser61) Polyclonal Antibody, Cy3 Conjugated
-
Category
Conjugated Primary Antibodies
-
Conjugation
Cy3
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
Bid Ser61
-
Specificity
This is a highly specific antibody against Bid Ser61.
-
Modification
Phosphorylation
-
Modification site
Ser61
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Source
KLH conjugated synthetic phosphopeptide derived from mouse Bid around the phosphorylation site of Ser61
-
Gene ID number
12122
-
Tested applications
IF(IHC-P)
-
Recommended dilutions
IF(IHC-P)(1:50-200)
-
Crossreactivity
Mouse
-
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Antigen background
Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
-
Purification method
This antibody was purified via Protein A.
-
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
-
Excitation Emission
512,550nm/570,615nm
-
Synonyms
Bid phospho S61; p-Bid phospho S61; BH3 interacting domain death agonist; p22 BID; BID; BH3-interacting domain death agonist; AltName: BH3-interacting domain death agonist p15; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID.
-
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-Bid Ser61 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
-
Additional conjugation
cy3 conjugation kit
-
French translation
anticorps
-
Gene target
-
Short name
Anti-Bid Ser61
-
Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
-
Isotype
Immunoglobulin G (IgG)
-
Label
Cy3
-
Alternative name
Anti-Bid Ser61 PAb Cy3
-
Alternative technique
antibodies
-
Alternative to gene target
BH3 interacting domain death a this GO nist, FP497, BID and IDBG-1050 and ENSG00000015475 and 101929618,637, ubiquitin protein ligase binding, Plasma membranes, Bid and IDBG-184076 and ENSMUSG00000004446 and 12122, BID and IDBG-641320 and ENSBTAG00000013988 and 510373,782484
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products