BCAR1(Tyr410) Antibody, FITC Conjugated
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Catalog number
bs-6224R-FITC
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Price
Please ask
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Size
0.1ml
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Long name
BCAR1(Tyr410) Polyclonal Antibody, FITC Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
FITC
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
BCAR1 Tyr410
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Specificity
This is a highly specific antibody against BCAR1 Tyr410. Will recognize BCAR1(Tyr414) in mouse and BCAR1(Tyr508) in rat
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Modification
Phosphorylation
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Modification site
Tyr410
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
405-420/870
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Subcellular location
Cytoplasm, Cell membrane
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Source
KLH conjugated synthetic phosphopeptide derived from human BCAR1 around the phosphorylation site of Tyr410.
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Gene ID number
9564
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Swiss Prot
P56945
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
494nm/518nm
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Synonyms
CAS; CAS1; CASS1; CRKAS; P130Cas; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; BCAR1
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
BCAR1
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Short name
Anti-BCAR1 Tyr410
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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Isotype
Immunoglobulin G (IgG)
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Label
FITC
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Alternative name
Anti-BCAR1 Tyr410 PAb FITC
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Alternative technique
antibodies, fluorescine
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Alternative to gene target
breast cancer anti-estrogen resistance 1, CAS and CAS1 and CASS1 and CRKAS and P130Cas, BCAR1 and IDBG-42468 and ENSG00000050820 and 9564, protein kinase binding, nuclei, Bcar1 and IDBG-193577 and ENSMUSG00000031955 and 12927, BCAR1 and IDBG-629067 and ENSBTAG00000012002 and
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Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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