BAD (Ser99) Antibody, Cy5 Conjugated
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Catalog number
bs-5219R-Cy5
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Price
Please ask
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Size
0.1ml
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Long name
BAD (Ser99) Polyclonal Antibody, Cy5 Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Cy5
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
BAD Ser99
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Specificity
This is a highly specific antibody against BAD Ser99.
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Modification
Phosphorylation
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Modification site
Ser99
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
KLH conjugated synthetic phosphopeptide derived from human BAD around the phosphorylation site of Ser99
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Gene ID number
572
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
625,650nm/670nm
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Synonyms
Bad phospho S99; Bad phospho Ser99; p-Bad S99;p- Bad Ser99; p-Bad phospho Ser99; BBC 2; BBC2; BBC6; Bcl 2 Antagonist of Cell Death; Bcl 2 Binding Component 6; BCL X / BCL 2 Binding Protein; BCL X Binding Protein; Bcl XL/Bcl 2 Associated Death Promoter; Bcl-2-like protein 8; Bcl2 antagonist of cell death; BCL2 antagonist of cell death protein; BCL2 associated agonist of cell death; Bcl2 Associated Death Promoter; BCL2 binding component 6; BCL2 binding protein; Bcl2 Like 8 Protein; Bcl2-L-8; BCL2L8; BclXL; Proapoptotic BH3 Only Protein; BAD_HUMAN; Bcl-2-binding component 6.
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
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Additional conjugation
Cy5.5
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French translation
anticorps
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Gene target
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Gene symbol
BAD, LAMP5
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Short name
Anti-BAD Ser99
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Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
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Isotype
Immunoglobulin G (IgG)
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Label
Cy5
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Alternative name
Anti-BAD Ser99 PAb Cy5
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Alternative technique
antibodies
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Alternative to gene target
BCL2-associated a this GO nist of cell death, BBC2 and BCL2L8, BAD and IDBG-53423 and ENSG00000002330 and 572, protein heterodimerization activity, Cytoplasm, Bad and IDBG-137335 and ENSMUSG00000024959 and 12015, BT.55802 and IDBG-643918 and ENSBTAG00000012511 and 615013
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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