ATP1A1 (Tyr10) Antibody, FITC Conjugated

• Catalog number
  bs-3034R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  ATP1A1 (Tyr10) Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  ATP1A1 Tyr10
• Specificity
  This is a highly specific antibody against ATP1A1 Tyr10.
• Modification
  Phosphorylation
• Modification site
  Tyr10
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  1-30/1023
• Subcellular location
  Cytoplasm
• Source
  KLH conjugated synthetic phosphopeptide derived from human Na,K-ATPase alpha-1 around the phosphorylation site of tyrosine 10
• Gene ID number
  24211
• Swiss Prot
  P06685
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  Nkaa1b; Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1; Atp1a1
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  ATP1A1   Tyr10   Conjugated  
• Gene symbol
  ATP1A1, ATP1A1-AS1
• Short name
  Anti-ATP1A1 Tyr10
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-ATP1A1 Tyr10 PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:799
• Gene
  ATP1A1
• Long gene name
  ATPase Na+/K+ transporting subunit alpha 1
• Synonyms gene name
  • ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Synonyms name
  • sodium/potassium-transporting ATPase subunit alpha-1
  • sodium pump subunit alpha-1
  • sodium-potassium ATPase catalytic subunit alpha-1
• GenBank acession
  • D00099
• Locus
  1p13.1
• Discovery year
  1988-05-11
• Entrez gene record
  476
• RefSeq identity
  • NM_001160233
• Classification
  • ATPase Na+/K+ transporting subunits
• VEGA ID
  OTTHUMG00000012109

Gene info

• Identity
  HGNC:28262
• Gene
  ATP1A1-AS1
• Long gene name
  ATP1A1 antisense RNA 1
• Synonyms gene
  • C1orf203
  • ATP1A1OS
• Synonyms gene name
  • chromosome 1 open reading frame 203
  • ATP1A1 opposite strand
• Synonyms
  • MGC16179
• GenBank acession
  • BC007373
• Locus
  1p13.1
• Discovery year
  2005-11-17
• Entrez gene record
  84852
• Pubmed identfication
  • 12477932
• RefSeq identity
  • NR_024124
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000012111

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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