ATG1(Ser556) Antibody, FITC Conjugated

• Catalog number
  bs-3464R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  ATG1(Ser556) Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  ATG1 Ser556
• Specificity
  This is a highly specific antibody against ATG1 Ser556.
• Modification
  Phosphorylation
• Modification site
  Ser556
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  530-580/1050
• Subcellular location
  Cytoplasm
• Source
  KLH conjugated synthetic phosphopeptide derived from human ATG1 around the phosphorylation site of Ser556
• Gene ID number
  8408
• Swiss Prot
  O75385
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  ATG1; ATG1A; UNC51; hATG1; Unc51.1; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; Unc-51-like kinase 1; ULK1; KIAA0722
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  ATG1   Ser556   Conjugated  
• Gene symbol
  ULK1
• Short name
  Anti-ATG1 Ser556
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-ATG1 Ser556 PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:12558
• Gene
  ULK1
• Long gene name
  unc-51 like autophagy activating kinase 1
• Synonyms gene name
  • unc-51 (C. elegans)-like kinase 1
  • unc-51-like kinase 1 (C. elegans)
• Synonyms
  • ATG1
  • ATG1A
• Synonyms name
  • ATG1 autophagy related 1 homolog (S. cerevisiae)
• GenBank acession
  • AF045458
• Locus
  12q24.33
• Discovery year
  1998-09-03
• Entrez gene record
  8408
• Pubmed identfication
  • 9693035
• Classification
  • Autophagy related
• VEGA ID
  OTTHUMG00000168052

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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