ARP2/ARP3 Antibody, FITC Conjugated
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Catalog number
bs-12524R-FITC
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Price
Please ask
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Size
0.1ml
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Long name
ARP2/ARP3 Polyclonal Antibody, FITC Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
FITC
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
ARP2/ARP3
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Specificity
This is a highly specific antibody against ARP2/ARP3.
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Modification
Unmodified
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Modification site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
KLH conjugated synthetic peptide derived from human ARP2/ARP3
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Gene ID number
10097
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping of existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
494nm/518nm
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Synonyms
Actin like protein 2; Actin related protein 2; Actin-like protein 2; ACTIN-RELATED PROTEIN 2; ACTR2; ARP2 actin related protein 2 homolog yeast; ARP2; ARP2/3 COMPLEX; ARP2_HUMAN; OTTHUMP00000159937; OTTHUMP00000202270.
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
ACTR3, ACTR2, APOBEC3A, ANGPTL2, AP4M1, AICDA
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Short name
Anti-ARP2/ARP3
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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Isotype
Immunoglobulin G (IgG)
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Label
FITC
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Alternative name
Anti-ARP2/ARP3 PAb FITC
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Alternative technique
antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene name
actin related protein 3
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Synonyms gene name
- ARP3 (actin-related protein 3, yeast) homolog
- ARP3 actin-related protein 3 homolog (yeast)
- ARP3 actin related protein 3 homolog
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-05-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Actin related protein 2/3 complex subunits
- Actin related proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
actin related protein 2
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Synonyms gene name
- ARP2 (actin-related protein 2, yeast) homolog
- ARP2 actin-related protein 2 homolog (yeast)
- ARP2 actin related protein 2 homolog
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-05-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Actin related protein 2/3 complex subunits
- Actin related proteins
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
angiopoietin like 2
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-02-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Angiopoietin like family
- Fibrinogen C domain containing
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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