APG5L Antibody, Biotin Conjugated

• Catalog number
  bs-4005R-Biotin
• Price
  Please ask
• Size
  0.1ml

• Long name
  APG5L Polyclonal Antibody, Biotin Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Biotin
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  APG5L
• Specificity
  This is a highly specific antibody against APG5L.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  118-158/275
• Subcellular location
  Cytoplasm, Membrane bound
• Source
  KLH conjugated synthetic peptide derived from human APG5L
• Gene ID number
  9474
• Swiss Prot
  Q9H1Y0
• Tested applications
  WB, IHC-P
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG1 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT2 via the autophagic pathway. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  N/A
• Synonyms
  ASP; APG5; APG5L; hAPG5; APG5-LIKE; Autophagy protein 5; Apoptosis-specific protein; ATG5
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Additional conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  APG5L   Conjugated  
• Gene symbol
  ATG5
• Short name
  Anti-APG5L
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Biotin
• Alternative name
  Anti-APG5L PAb Biotin
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:589
• Gene
  ATG5
• Long gene name
  autophagy related 5
• Synonyms gene
  • APG5L
• Synonyms gene name
  • APG5 (autophagy 5, S. cerevisiae)-like
  • APG5 autophagy 5-like (S. cerevisiae)
  • ATG5 autophagy related 5 homolog (S. cerevisiae)
• Synonyms
  • ASP
  • APG5
  • hAPG5
• GenBank acession
  • Y11588
• Locus
  6q21
• Discovery year
  1999-09-16
• Entrez gene record
  9474
• Pubmed identfication
  • 9563500
  • 11349150
  • 15778222
• RefSeq identity
  • NM_004849
• Classification
  • Autophagy related
• VEGA ID
  OTTHUMG00000016193

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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