AMPK alpha-1/2 (Thr183/Thr172) Antibody, Cy3 Conjugated

• Catalog number
  bs-4002R-Cy3
• Price
  Please ask
• Size
  0.1ml

• Long name
  AMPK alpha-1/2 (Thr183/Thr172) Antibody, Cy3 Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Cy3
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  AMPK alpha-1/2 Thr183/Thr172
• Specificity
  This is a highly specific antibody against AMPK alpha-1/2 Thr183/Thr172.
• Modification
  Phosphorylation
• Modification site
  Thr172/Thr183
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Subcellular location
  Cytoplasm, Nucleus
• Source
  KLH conjugated synthetic phosphopeptide derived from mouse AMPK alpha 2 around the phosphorylation site of Thr172
• Gene ID number
  5562
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  512,550nm/570,615nm
• Synonyms
  PRKAA1phospho T172; AMPK alpha 1 + AMPK alpha 2 phospho T183/T172; 5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; AAPK1; acetyl CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha -1; AMPK 1; AMPK alpha 1 chain; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116; EC 2.7.11.1; HMG CoA reductase kinase;hormone sensitive lipase kinase; im:7154392; kinase AMPK alpha1; MGC33776; MGC57364; PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; wu:fa94c10.
• Description
  The Anti-AMPK alpha-1/2 Thr183/Thr172 is a α- or alpha protein sometimes glycoprotein present in blood.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-AMPK alpha-1/2 Thr183/Thr172 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
• Additional conjugation
  cy3 conjugation kit
• French translation
  anticorps

• Gene target
  AMPK   alpha-1/2   Thr183/Thr172   Conjugated  
• Gene symbol
  PRKAA2
• Short name
  Anti-AMPK alpha-1/2 Thr183/Thr172
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Cy3
• Alternative name
  Anti-AMPK alpha-1/2 Thr183/Thr172 PAb Cy3
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:9377
• Gene
  PRKAA2
• Long gene name
  protein kinase AMP-activated catalytic subunit alpha 2
• Synonyms gene
  • PRKAA
• Synonyms gene name
  • protein kinase, AMP-activated, alpha 2 catalytic subunit
• Synonyms
  • AMPK
  • AMPKa2
• GenBank acession
  • BC069823
• Locus
  1p32.2
• Discovery year
  1995-12-12
• Entrez gene record
  5563
• Pubmed identfication
  • 7959015
• RefSeq identity
  • NM_006252
• VEGA ID
  OTTHUMG00000008282

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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