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Long name
pan Cytokeratin Polyclonal Antibody, ALEXA FLUOR 555 Conjugated
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Also known as
Anti-pan Cytokeratin PAb ALEXA FLUOR 555
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 555
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
pan Cytokeratin
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Specificity
This is a highly specific antibody against pan Cytokeratin.
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Modification
Unmodified
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Modification Site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen range
170-210/644
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Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human pan cytokeratin
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Tested applications
IF(IHC-P), IF(IHC-F)
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Recommended dilutions
IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200)
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Crossreactivity
Human, Mouse, Rat, Pig
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes. In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane. Numerous accessory proteins are involved in the genesis and maintenance of such structure. This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms. Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, cell movement and differentiation. Cytokeratins interact with desmosomes and hemidesmosomes, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection.
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
553nm/568nm
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Synonyms
pan-cytokeratin; pan-CK; pan CK; P-CK; wide spectrum Cytokeratin; Cytokeratins; [cytokeratins 1, 2,4,5,6,7,8,71,72,75,78].
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Properties
For facs or microscopy Alexa 1 conjugate. Very high photo stable ALEXA conjugate. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor, ALEXA FLUOR 555
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French translation
anticorps
