NEDD4 Antibody, ALEXA FLUOR 488

• Catalog number
  bs-7877R-A488
• Price
  Please ask
• Size
  100ul

• Long name
  NEDD4 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
• Also known as
  Anti-NEDD4 PAb ALEXA FLUOR 488
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 488
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  NEDD4
• Specificity
  This is a highly specific antibody against NEDD4.
• Modification
  Unmodified
• Modification Site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  861-890/1319
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human NEDD4
• Gene ID Number
  4734
• Swiss Prot
  P46934
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding.
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  499nm/519nm
• Synonyms
  RPF1; NEDD4-1; E3 ubiquitin-protein ligase NEDD4; Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4; NEDD-4; NEDD4; KIAA0093; PIG53
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-NEDD4 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor
• French translation
  anticorps

• Gene target
  NEDD4  
• Gene symbol
  NEDD4, NEDD4L
• Short name
  Anti-NEDD4
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 488
• Alternative name
  NEDD4 Polyclonal Antibody, ALEXA FLUOR 488
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:7727
• Gene
  NEDD4
• Long gene name
  NEDD4 E3 ubiquitin protein ligase
• Synonyms gene name
  • neural precursor cell expressed, developmentally down-regulated 4
  • neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
• Synonyms
  • KIAA0093
  • MGC176705
  • NEDD4-1
  • RPF1
• Synonyms name
  • receptor-potentiating factor 1
• GenBank acession
  • D42055
• Locus
  15q21.3
• Discovery year
  1994-01-21
• Entrez gene record
  4734
• Pubmed identfication
  • 9073511
  • 8649367
• RefSeq identity
  • NM_198400
• Classification
  • HECT domain containing
• VEGA ID
  OTTHUMG00000132015

Gene info

• Identity
  HGNC:7728
• Gene
  NEDD4L
• Long gene name
  NEDD4 like E3 ubiquitin protein ligase
• Synonyms gene name
  • neural precursor cell expressed, developmentally down-regulated 4-like
  • neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
• Synonyms
  • KIAA0439
  • RSP5
  • NEDD4-2
• GenBank acession
  • AF210730
• Locus
  18q21.31
• Discovery year
  2000-03-14
• Entrez gene record
  23327
• Pubmed identfication
  • 10594025
  • 11244092
  • 18322022
• Classification
  • HECT domain containing
  • C2 domain containing
• VEGA ID
  OTTHUMG00000179875

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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