Lefty Antibody, ALEXA FLUOR 488
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Catalog numberbs-11236R-A488
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Price:
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Size100ul
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Long nameLefty Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
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Also known asAnti-Lefty PAb ALEXA FLUOR 488
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CategoryConjugated Primary Antibodies
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Conjugated withALEXA FLUOR® 488
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenLefty
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SpecificityThis is a highly specific antibody against Lefty.
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ModificationUnmodified
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Modification SiteNone
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Lefty
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Gene ID Number10637
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityHuman, Mouse, Rat
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigenDuring vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
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PurificationPurified by Protein A.
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Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission499nm/519nm
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SynonymsProtein lefty-1; AI450052; Ebaf; Left right determination factor 1; left right determination factor; Left right determination factor B; left-right determination factor; LEFT-RIGHT DETERMINATION FACTOR 1; LEFTY1; left-right determination, factor B; LEFT-RIGHT DETERMINATION, FACTOR B; LEFTY B; LEFTB; LEFTY 1; Lefty; lefty-1; LEFTY1; LEFTY1, MOUSE, HOMOLOG OF antibody; LEFTYB; Protein lefty-B; RGD1561867; Stimulated by retinoic acid gene 3 protein; STRA3; TGF beta 4; TGF-beta-4; Tgfb4; Transforming growth factor beta 4; Transforming growth factor beta-4.
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PropertiesFor facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Lefty has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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ConjugationAlexa Fluor
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French translationanticorps
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Gene target
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Gene symbolLEFTY2
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Short nameAnti-Lefty
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 488
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Alternative nameLefty Polyclonal Antibody, ALEXA FLUOR 488
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameleft-right determination factor 2
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Synonyms gene
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Synonyms gene name
- endometrial bleeding associated factor (left-right determination, factor A; transforming growth factor beta superfamily)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-01-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Transforming growth factor beta superfamily
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data