Histone H3 (acetyl K9) Antibody, ALEXA FLUOR 488

  • Catalog number
    bs-3776R-A488
  • Price
    Please ask
  • Size
    100ul
  • Long name
    Histone H3 (acetyl K9) Antibody, ALEXA FLUOR 488 Conjugated
  • Also known as
    Anti-Histone H3 acetyl K9 PAb ALEXA FLUOR 488
  • Category
    Conjugated Primary Antibodies
  • Conjugated with
    ALEXA FLUOR® 488
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    Histone H3 acetyl K9
  • Specificity
    This is a highly specific antibody against Histone H3 acetyl K9.
  • Modification
    Acetylation
  • Modification Site
    acetyl K9
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Concentration
    1ug per 1ul
  • Immunogen range
    1-40/136
  • Subcellular location
    Nucleus
  • Source
    KLH conjugated synthetic acetylpeptide derived from human Histone H3 around acetylation site of Lys9
  • Gene ID Number
    3020
  • Swiss Prot
    P84243
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Cross reactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Background of the antigen
    Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Purification
    Purified by Protein A.
  • Storage conditions
    Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
  • Excitation emission
    499nm/519nm
  • Synonyms
    H3F3; H3.3A; Histone H3.3; H3F3A; PP781
  • Description
    N terminal acetylation or CH3CO as epigenetic regulation of Anti-Histone H3 acetyl K9 by NATs.
  • Properties
    For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Histone H3 acetyl K9 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Conjugation
    Alexa Fluor
  • French translation
    anticorps
  • Gene target
    Histone  
  • Short name
    Anti-Histone H3 acetyl K9
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Immunoglobulin G (IgG)
  • Label
    ALEXA FLUOR 488
  • Alternative name
    Histone H3 (acetyl K9) Antibody, ALEXA FLUOR 488
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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