hHBrk1 Antibody, ALEXA FLUOR 488
-
Catalog number
bs-5124R-A488
-
Price
Please ask
-
Size
100ul
-
-
Long name
hHBrk1 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
-
Also known as
Anti-hHBrk1 PAb ALEXA FLUOR 488
-
Category
Conjugated Primary Antibodies
-
Conjugated with
ALEXA FLUOR® 488
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
hHBrk1
-
Specificity
This is a highly specific antibody against hHBrk1.
-
Modification
Unmodified
-
Modification Site
None
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human hHBrk1
-
Gene ID Number
55845
-
Tested applications
IF(IHC-P)
-
Recommended dilutions
IF(IHC-P)(1:50-200)
-
Crossreactivity
Human, Mouse, Rat
-
Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Background of the antigen
HSPC300 (hematopoietic stem cell protein 300) is also known as probable protein BRICK1 or C3orf10 (chromosome 3 open reading frame 10) and is a 75 amino acid protein that is expressed as two isoforms and localizes to both the cytoplasm and the cytoskeleton. HSPC300 is thought to regulate cytoskeletal organization and Actin polymerization. Free HSPC300 exists as homotrimers prior to its incorporation into the WAVE complex. The WAVE complex includes five proteins, one of which is HSPC300, that regulate the ARC (Arp2/3 complex) which is responsible for Actin nucleation and is Rac 1-dependent. Because HSPC300 is a highly conserved subunit of the WAVE complex across many species, it is thought to have the same or similar functions in many different organisms. In Drosophila, the WAVE/ARC pathway may affect the development of the nervous system. HSPC300 is thought to localize to axons of the central nervous system of Drosophila embryos and thus may also be involved in axonogenesis. In addition, HSPC300 is thought to be necessary for synaptic morphogenesis by motoneurons. In mice, the knockout of the WAVE complex leads to learning and memory defects, and it is therefore hypothesized that HSPC300 may also be involved in cognitive functions. Genetic depletion of HSPC300 results in cytoskeletal abnormalities and prevents cytokinesis of cells, suggesting that decreased levels of HSPC300 may be associated with tumor suppression.
-
Purification
Purified by Protein A.
-
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
-
Excitation emission
499nm/519nm
-
Synonyms
Brk1-like; C3orf10 chromosome 3 open reading frame 10; C3ORF10 GENE; Chromosome 3 open reading frame 10; hHBrk1; HSPC300; MDS027; Probable protein BRICK1; BRK1_HUMAN.
-
Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-hHBrk1 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Conjugation
Alexa Fluor
-
French translation
anticorps
-
Gene target
-
Short name
Anti-hHBrk1
-
Technique
Antibody, antibodies against human proteins, antibodies for
-
Isotype
Immunoglobulin G (IgG)
-
Label
ALEXA FLUOR 488
-
Alternative name
hHBrk1 Polyclonal Antibody, ALEXA FLUOR 488
-
Alternative technique
antibodies
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products