Calpain 2 Antibody, ALEXA FLUOR 488

• Catalog number
  bs-1599R-A488
• Price
  Please ask
• Size
  100ul

• Long name
  Calpain 2 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
• Also known as
  Anti-Calpain 2 PAb ALEXA FLUOR 488
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 488
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  Calpain 2
• Specificity
  This is a highly specific antibody against Calpain 2.
• Modification
  Unmodified
• Modification Site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic peptide derived from mouse Calpain 2
• Gene ID Number
  824
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  499nm/519nm
• Synonyms
  Calcium activated neutral proteinase 2; Calcium activated neutral proteinase; CALP80; Calpain 2 m/II large subunit; Calpain 2 catalytic subunit; Calpain 2 large catalytic subunit; Calpain 2 large subunit; Calpain large polypeptide L2; Calpain M type; Calcium-activated neutral proteinase 2; CALP80; Calpain 2, large [catalytic] subunit; Calpain M-type; Calpain-2 catalytic subunit; Calpain-2 large subunit; CAN2_MOUSE; M-calpain; Millimolar-calpain; Calpain2; CANP 2; CANP L2; CANP2; CANPL 2; CANPL2; CANPml; Capa2; CAPN 2; CAPN2; FLJ39928; M calpain; M calpin; M type; mCANP; Millimolar calpain.
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Calpain 2 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor
• French translation
  anticorps

• Gene target
  Calpain  
• Gene symbol
  CAPN8, CAPN2, CAPNS2
• Short name
  Anti-Calpain 2
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 488
• Alternative name
  Calpain 2 Polyclonal Antibody, ALEXA FLUOR 488
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:1485
• Gene
  CAPN8
• Long gene name
  calpain 8
• Synonyms gene name
  • calpain 8 (nCL-2)
• Synonyms
  • nCL-2
• Locus
  1q41
• Discovery year
  1998-09-25
• Entrez gene record
  388743
• Pubmed identfication
  • 7690035
  • 8889549
• RefSeq identity
  • NM_001143962
• Classification
  • Calpains
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000037378

Gene info

• Identity
  HGNC:1479
• Gene
  CAPN2
• Long gene name
  calpain 2
• Synonyms gene name
  • calpain 2, (m/II) large subunit
• Synonyms
  • mCANP
  • CANPml
  • CANPL2
• GenBank acession
  • J04700
• Locus
  1q41
• Discovery year
  1989-06-30
• Entrez gene record
  824
• Pubmed identfication
  • 2852952
  • 2539381
• RefSeq identity
  • NM_001748
• Classification
  • EF-hand domain containing
  • Calpains
• VEGA ID
  OTTHUMG00000037376

Gene info

• Identity
  HGNC:16371
• Gene
  CAPNS2
• Long gene name
  calpain small subunit 2
• Synonyms
  • MGC12536
  • MGC14804
• GenBank acession
  • AY052551
• Locus
  16q12.2
• Discovery year
  2004-07-19
• Entrez gene record
  84290
• Pubmed identfication
  • 11853546
• RefSeq identity
  • NM_032330
• Classification
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000167812

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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