ATF2(Thr71) Antibody, ALEXA FLUOR 488
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Catalog numberbs-12540R-A488
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Price:
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Size100ul
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Long nameATF2(Thr71) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
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Also known asAnti-ATF2 Thr71 PAb ALEXA FLUOR 488
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CategoryConjugated Primary Antibodies
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Conjugated withALEXA FLUOR® 488
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenATF2 Thr71
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SpecificityThis is a highly specific antibody against ATF2 Thr71.
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ModificationPhosphorylation
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Modification SiteThr71
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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SourceKLH conjugated synthetic phosphopeptide derived from human ATF2 around the phosphorylation site of Thr71
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Gene ID Number1386
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityHuman
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigenATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
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PurificationPurified by Protein A.
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Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission499nm/519nm
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SynonymsATF2 phospho T71; p-ATF2 phospho T71; ATF2 phospho Thr71; p-ATF2 phospho Thr71; CREB 2; HB 16;Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_HUMAN.
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PropertiesFor facs or microscopy Alexa 1 conjugate. Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-ATF2 Thr71 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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ConjugationAlexa Fluor
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French translationanticorps
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Gene target
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Gene symbolGDNF, ATF2
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Short nameAnti-ATF2 Thr71
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 488
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Alternative nameATF2(Thr71) Polyclonal Antibody, ALEXA FLUOR 488
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameglial cell derived neurotrophic factor
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Synonyms
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Synonyms name
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Locus
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Discovery year1995-05-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- GDNF family ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameactivating transcription factor 2
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Synonyms gene
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Synonyms gene name
- cAMP responsive element binding protein 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-08-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Basic leucine zipper proteins
- MicroRNA protein coding host genes
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data