A Raf (Tyr301 + Tyr302) Antibody, ALEXA FLUOR 647
-
Catalog number
bs-3421R-A647
-
Price
Please ask
-
Size
100ul
-
-
Long name
A Raf (Tyr301 + Tyr302) Antibody, ALEXA FLUOR 647 Conjugated
-
Also known as
Anti-A Raf Tyr301 + Tyr302 PAb ALEXA FLUOR 647
-
Category
Conjugated Primary Antibodies
-
Conjugated with
ALEXA FLUOR® 647
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
A Raf Tyr301 + Tyr302
-
Specificity
This is a highly specific antibody against A Raf Tyr301 + Tyr302.
-
Modification
Phosphorylation
-
Modification Site
Tyr301 + Tyr302
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Source
KLH conjugated synstic phosphopeptide derived from human A Raf around the phosphorylation site of Tyr301/302 [G(p-Y)(p-Y)WE]
-
Gene ID Number
369
-
Tested applications
IF(IHC-P)
-
Recommended dilutions
IF(IHC-P)(1:50-200)
-
Crossreactivity
Human, Mouse, Rat
-
Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Background of the antigen
The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
-
Purification
Purified by Protein A.
-
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
-
Excitation emission
650nm/665nm
-
Synonyms
A RAF phospho Y301 + Y302; p-A RAF phospho Y301 + Y302; A raf 1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; ARAF; ARaf proto oncogene serine/threonine protein kinase; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1 murine sarcoma 3611 virus; ARAF_HUMAN.
-
Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-A Raf Tyr301 + Tyr302 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Conjugation
Alexa Fluor, ALEXA FLUOR 647
-
French translation
anticorps
-
Gene target
-
Short name
Anti-A Raf Tyr301 + Tyr302
-
Technique
Antibody, antibodies against human proteins, antibodies for
-
Isotype
Immunoglobulin G (IgG)
-
Label
ALEXA FLUOR 647
-
Alternative name
A Raf (Tyr301 + Tyr302) Antibody, ALEXA FLUOR 647
-
Alternative technique
antibodies
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products