Anti-Ube2L3 (Polyclonal), ALEXA Fluor 594
-
Catalog numberGENTObs-8350R-A594
-
PricePlease ask
-
Size100 microliters
-
-
TypeConjugated Primary Antibody
-
Conjugated withALEXA FLUOR® 594
-
Host organismRabbit (Oryctolagus cuniculus)
-
Target Protein PeptideUbe2L3
-
SpecificityThis antibody reacts specifically with Ube2L3
-
ModificationNo modification has been applied to this antibody
-
Modification siteNone
-
ClonalityPolyclonal Antibody
-
ClonePolyclonal Antibodies
-
Concentration1ug per 1ul
-
Subcellular locationsN/A
-
Antigen SourceKLH conjugated synthetic peptide derived from human Ube2L3
-
Gene ID7332
-
Swiss ProtN/A
-
ApplicationsIF(IHC-P)
-
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
-
Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
-
Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
-
Background informationThe ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2E1 and UBE2L3, also designated UBCH6 and UBCH7 respectively in human, are E2 conjugating enzymes that interact with various proteins. Specifically, UBE2E1 interacts with the tumor suppressor protein TSSC5. UBE2L3 has been shown to mediate c-fos degradation, NF-kB maturation, human papilloma virus-mediated p53 and Myc protein degradation.
-
Purification methodPurified by Protein A.
-
StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
-
Excitation emission590nm/617nm
-
SynonymsE2 F1; E2-F1; L UBC; L-UBC; UB2L3_HUMAN; UBCE7; UbcH7; UbcM4; Ube2l3; Ubiquitin carrier protein L3; Ubiquitin conjugating enzyme E2 L3; Ubiquitin protein ligase L3; Ubiquitin-conjugating enzyme E2 L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3.
-
Also known asUbe2L3 Polyclonal Antibody
-
Other nameAnti-Ube2L3 Polyclonal
-
AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
-
PropertiesFor facs or microscopy Alexa 1 conjugate.
-
ConjugationAlexa Fluor, ALEXA FLUOR® 594
-
ConjugatedAlexa conjugate 1
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
GroupPolyclonals and antibodies
-
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
Gene target
-
Gene symbolUBE2L3
-
Short nameAnti-Ube2L3
-
TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
IsotypeImmunoglobulin G (IgG)
-
LabelALEXA FLUOR 594
-
Alternative nameUbe2L3 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
-
Alternative techniquepolyclonals, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameubiquitin conjugating enzyme E2 L3
-
Synonyms gene name
- ubiquitin-conjugating enzyme E2L 3
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1997-03-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Ubiquitin conjugating enzymes E2
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data