-
Type
Conjugated Primary Antibody
-
Conjugated with
ALEXA FLUOR® 594
-
Host organism
Rabbit (Oryctolagus cuniculus)
-
Target Protein Peptide
SYVN1
-
Specificity
This antibody reacts specifically with SYVN1
-
Modification
No modification has been applied to this antibody
-
Modification site
None
-
Clonality
Polyclonal Antibody
-
Clone
Polyclonal Antibodies
-
Concentration
1ug per 1ul
-
Subcellular locations
Cytoplasm
-
Antigen Source
KLH conjugated synthetic peptide derived from human SYVN1
-
Gene ID
84447
-
Swiss Prot
Q86TM6
-
Applications
IF(IHC-P), IF(IHC-F)
-
Applications with corresponding dilutions
IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200)
-
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
-
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
-
Background information
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
-
Purification method
Purified by Protein A.
-
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
-
Excitation emission
590nm/617nm
-
Synonyms
DER3; HRD1; E3 ubiquitin-protein ligase synoviolin; Synovial apoptosis inhibitor 1; SYVN1; KIAA181
-
Also known as
SYVN1 Polyclonal Antibody
-
Other name
Anti-SYVN1 Polyclonal
-
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
-
Properties
For facs or microscopy Alexa 1 conjugate.
-
Conjugation
Alexa Fluor, ALEXA FLUOR® 594
-
Conjugated
Alexa conjugate 1
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
Gene target
-
Gene symbol
SYVN1
-
Short name
Anti-SYVN1
-
Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Isotype
Immunoglobulin G (IgG)
-
Label
ALEXA FLUOR 594
-
Alternative name
SYVN1 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
-
Alternative technique
polyclonals, antibodies
-
Alternative to gene target
synovial apoptosis inhibitor 1, synoviolin, SYVN1 and IDBG-56611 and ENSG00000162298 and 102465449,84447, metal ion binding, nuclei, Syvn1 and IDBG-134090 and ENSMUSG00000024807 and 74126, BT.54995 and IDBG-644142 and ENSBTAG00000005076 and 508358
-
