Anti-SHC (Tyr427) (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-3412R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  SHC Tyr427
• Specificity
  This antibody reacts specifically with SHC (Tyr427)
• Modification
  Phosphorylation
• Modification site
  Tyr427
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic phosphopeptide derived from human SHC around the phosphorylation site of Tyr427
• Gene ID
  6464
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  The SHC gene encodes for a signaling and transforming protein containing Src homology 2 and 3 (SH2 and SH3) domains. The SHC gene encodes 2 widely expressed overlapping proteins of 46 and 52 kD, both containing a C-terminal SH2 domain. Adjacent to the SH2 region is a glycine and proline rich region. These 2 proteins differ in their N terminals. SHC proteins are involved in mitogenic signal transduction and act by coupling growth factor receptors to the RAS signaling pathway. The protein encoded by the SHC1 gene is thought to act as an adaptor in many signal transduction pathways.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  Shc1Tyr427; p46; p52; p52 SHC; p52SHC; p66; p66 SHC; p66SHC; SH2 domain protein C1; SHC Src homology 2 domain containing transforming protein 1; SHC 1; SHC A; Shc; SHC transforming protein 1; SHC transforming protein; SHC1; SHCA; Src homology 2 domain containing transforming protein C1.
• Also known as
  SHC (Tyr427) Polyclonal Antibody
• Other name
  Anti-SHC (Tyr427) Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  SHC   Tyr427  
• Gene symbol
  SHC1, SHC3
• Short name
  Anti-SHC (Tyr427)
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  SHC (Tyr427) Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:10840
• Gene
  SHC1
• Long gene name
  SHC adaptor protein 1
• Synonyms gene
  • SHC
• Synonyms gene name
  • SHC (Src homology 2 domain-containing) transforming protein 1
• Synonyms
  • p66
  • ShcA
• GenBank acession
  • U73377
• Locus
  1q21.3
• Discovery year
  1993-02-17
• Entrez gene record
  6464
• Pubmed identfication
  • 1623525
• RefSeq identity
  • NM_183001
• Classification
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000037295

Gene info

• Identity
  HGNC:18181
• Gene
  SHC3
• Long gene name
  SHC adaptor protein 3
• Synonyms gene name
  • src homology 2 domain containing transforming protein C3
  • SHC (Src homology 2 domain containing) transforming protein 3
• Synonyms
  • N-Shc
  • NSHC
  • SHCC
• GenBank acession
  • D84361
• Locus
  9q22.1
• Discovery year
  2003-11-12
• Entrez gene record
  53358
• Pubmed identfication
  • 8808684
• RefSeq identity
  • NM_016848
• Classification
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000020179

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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