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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR® 594
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
RPS6KA1 Ser352
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Specificity
This antibody reacts specifically with RPS6KA1(Ser352)
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Modification
Phosphorylation
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Modification site
Ser352
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
N/A
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Antigen Source
KLH conjugated synthetic phosphopeptide derived from human RPS6KA1 around the phosphorylation site of Ser352
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Gene ID
6195
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Swiss Prot
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
Rsk1 is a member of a family of 90kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c Fos and CREB.
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
p90RSK Ser352; RPS6KA1phospho S352; p90 RSK1 ; p90rsk ; p90S6K ; pp90RSK1 ; Ribosomal protein S6 kinase 90kD 1 ; Ribosomal protein S6 kinase 90kD polypeptide 1 ; Ribosomal protein S6 kinase 90kDa polypeptide 1 ; Ribosomal protein S6 kinase alpha 1 ; Ribosomal protein S6 kinase polypeptide 1 ; Ribosomal S6 kinase 1 ; RPS6K1 alpha ; rps6ka ; Rps6ka1 ; RSK 1 ; RSK 1 p90 ; RSK ; RSK1 ; S6K alpha 1 ; 90 kDa ribosomal protein S6 kinase 1 ; dJ590P13.1 ribosomal protein S6 kinase, 90kD, polypeptide 1 ; dJ590P13.1 ; EC 2.7.11.1 ; HU 1 ; HU1 ; MAP kinase activated protein kinase 1a ; MAPKAP kinase 1A ; MAPKAPK1A ; MGC79981 ; Mitogen-activated protein kinase-activated protein kinase 1A; KS6A1_MOUSE.
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Also known as
RPS6KA1(Ser352) Polyclonal Antibody
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Other name
Anti-RPS6KA1(Ser352) Polyclonal
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbol
RPS6KA1
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Short name
Anti-RPS6KA1(Ser352)
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Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 594
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Alternative name
RPS6KA1(Ser352) Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
ribosomal protein S6 kinase, 90kDa, polypeptide 1, HU-1 and MAPKAPK1A and RSK and RSK1, RPS6KA1 and IDBG-94680 and ENSG00000117676 and 6195, transferase activity, nuclei, Rps6ka1 and IDBG-195412 and ENSMUSG00000003644 and 20111, RPS6KA1 and IDBG-645125 and ENSBTAG00000014447 and 533908
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