Anti-Reticulon 4 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-1315R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  Reticulon 4
• Specificity
  Based on the location of the target sequence this antibody will recognize all isoforms(Nogo-A, Nogo-B, and Nogo-C).Reticulon 4
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Cytoplasm
• Antigen Source
  KLH conjugated synthetic peptide derived from Mouse Reticulon 4
• Gene ID
  68585
• Swiss Prot
  Q99P72
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The reticulon protein is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Reticulon 4 regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. It regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. May inhibit BACE1 activity and amyloid precursor protein processing.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  Reticulon-4; Rtn4; Neurite outgrowth inhibitor; Nogo protein; Foocen; Neuroendocrine-specific protein; NSP; euroendocrine-specific protein C homolog; RTN-x; Reticulon-5; RTN 4A; Nogo-A; RTN-xL; RTN 4B; ASY; Nogo-B; RTN-xS; Foocen-M; RTN 4C; Nogo-C; Foocen-S; RTN4-B2; Rtn-T
• Also known as
  Reticulon 4 Polyclonal Antibody
• Other name
  Anti-Reticulon 4 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  Reticulon  
• Gene symbol
  RTN4IP1, RTN4R, RTN4RL1
• Short name
  Anti-Reticulon 4
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  Reticulon 4 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:18647
• Gene
  RTN4IP1
• Long gene name
  reticulon 4 interacting protein 1
• Synonyms
  • NIMP
• GenBank acession
  • AF336861
• Locus
  6q21
• Discovery year
  2002-05-23
• Entrez gene record
  84816
• VEGA ID
  OTTHUMG00000015304

Gene info

• Identity
  HGNC:18601
• Gene
  RTN4R
• Long gene name
  reticulon 4 receptor
• Synonyms
  • NOGOR
• Synonyms name
  • NOGO receptor
• GenBank acession
  • AF283463
• Locus
  22q11.21
• Discovery year
  2002-04-30
• Entrez gene record
  65078
• Pubmed identfication
  • 11201742
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000150572

Gene info

• Identity
  HGNC:21329
• Gene
  RTN4RL1
• Long gene name
  reticulon 4 receptor like 1
• Synonyms gene name
  • reticulon 4 receptor-like 1
• Synonyms
  • NGRH2
  • NgR3
  • DKFZp547J144
• Synonyms name
  • nogo-66 receptor homolog 2
• GenBank acession
  • AF532859
• Locus
  17p13.3
• Discovery year
  2004-01-30
• Entrez gene record
  146760
• RefSeq identity
  • NM_178568
• VEGA ID
  OTTHUMG00000180184

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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