Anti-Rad17 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-3556R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  Rad17
• Specificity
  This antibody reacts specifically with Rad17
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human Rad17
• Gene ID
  5884
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013].
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  RFC; CCYC; Cell cycle checkpoint protein; Cell cycle checkpoint protein RAD17; HRAD 17; hRad17; R24L; Rad 17; Rad 24; RAD1 S. pombe homolog; RAD17; RAD17 homolog; Rad17 like protein; RAD17_HUMAN; RAD17Sp; Rad24; RF C activator 1 homolog; RF C/activator 1 homolog; RF-C/activator 1 homolog.
• Also known as
  Rad17 Polyclonal Antibody
• Other name
  Anti-Rad17 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  Rad17  
• Gene symbol
  RAD17P2, RAD17, RAD17P1
• Short name
  Anti-Rad17
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  Rad17 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:9809
• Gene
  RAD17P2
• Long gene name
  RAD17 pseudogene 2
• Synonyms gene name
  • RAD17 (S. pombe) homolog, pseudogene 2
  • RAD17 homolog (S. pombe) pseudogene 2
• Synonyms
  • HRAD17P2
• GenBank acession
  • AJ131310
• Locus
  13q14.2
• Discovery year
  1999-02-15
• Entrez gene record
  9206
• Pubmed identfication
  • 9660800
• VEGA ID
  OTTHUMG00000016908

Gene info

• Identity
  HGNC:9807
• Gene
  RAD17
• Long gene name
  RAD17 checkpoint clamp loader component
• Synonyms gene name
  • RAD1 (S. pombe) homolog
  • RAD17 homolog (S. pombe)
• Synonyms
  • Rad24
  • RAD17Sp
  • CCYC
• GenBank acession
  • AF085736
• Locus
  5q13.2
• Discovery year
  1998-07-31
• Entrez gene record
  5884
• Pubmed identfication
  • 9869296
  • 9660800
• RefSeq identity
  • NM_133344
• VEGA ID
  OTTHUMG00000099357

Gene info

• Identity
  HGNC:9808
• Gene
  RAD17P1
• Long gene name
  RAD17 pseudogene 1
• Synonyms gene name
  • RAD17 (S. pombe) homolog, pseudogene 1
  • RAD17 homolog (S. pombe) pseudogene 1
• Synonyms
  • HRAD17P1
• GenBank acession
  • AJ131309
• Locus
  7p21.1
• Discovery year
  1999-02-15
• Entrez gene record
  9207
• VEGA ID
  OTTHUMG00000152462

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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