Anti-Prickle (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-12411R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptidePrickle
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SpecificityThis antibody reacts specifically with Prickle
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsN/A
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Antigen SourceKLH conjugated synthetic peptide derived from human Prickle
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Gene ID144165
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Swiss ProtN/A
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationPrickle1 is an 831 amino acid protein that contains one PET domain and three LIM zinc-binding domains and localizes to the cytoplasm, as well as to the nuclear membrane. Expressed at higher levels in placenta and at lower levels in liver, brain, kidney, lung and pancreas, Prickle1 is thought to function as a nuclear receptor that interacts with NRSF, a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). Defects in the gene encoding Prickle1 are associated with autosomal recessive progressive myoclonic epilepsy-1B, which is characterized by quick jerks of the arms, shoulders or legs. The gene encoding Prickle1 maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsFLJ31627; FLJ31937; MGC138902; MGC138903; EPM1B; Prickle 1; Prickle homolog 1 Drosophila; Prickle like protein 1; Prickle-like 1; Prickle1; REST-Interacting LIM Domain Protein; REST/NRSF interacting LIM domain protein 1; RILP; PRIC1_HUMAN.
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Also known asPrickle Polyclonal Antibody
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Other nameAnti-Prickle Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolPRICKLE2, PRICKLE3, PRICKLE1
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Short nameAnti-Prickle
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative namePrickle Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameprickle planar cell polarity protein 2
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Synonyms gene name
- prickle-like 2 (Drosophila)
- prickle homolog 2 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-02-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- LIM domain containing
- Prickle planar cell polarity proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprickle planar cell polarity protein 3
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Synonyms gene
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Synonyms gene name
- LIM domain only 6
- prickle homolog 3 (Drosophila)
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GenBank acession
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Locus
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Discovery year1997-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- LIM domain containing
- Prickle planar cell polarity proteins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameprickle planar cell polarity protein 1
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Synonyms gene name
- prickle-like 1 (Drosophila)
- prickle homolog 1 (Drosophila)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-02-10
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Entrez gene record
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Pubmed identfication
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Classification
- LIM domain containing
- Prickle planar cell polarity proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
