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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR® 594
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
PCYT2
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Specificity
This antibody reacts specifically with PCYT2
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Modification
No modification has been applied to this antibody
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Modification site
None
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
N/A
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Antigen Source
KLH conjugated synthetic peptide derived from human PCYT2
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Gene ID
N/A
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Swiss Prot
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
Phosphatidylethanolamine (PtdEtn) is a major membrane phospholipid which serves to play a primary role in cell membrane structure and is also involved in cell division, cell signaling, activation, phagocytosis and autophagy. PCYT2 (Phosphorylethanolamine transferase), also known as Ethanolamine-phosphate cytidylyltransferase, is a 389 amino acid protein that catalyzes the formation of CDP-ethanolamine from ethanolamine. This product combined with diacylglycerol form phosphatidylethanolamine via the de novo Kennedy pathway. PCYT2 is expressed at highest levels in heart, liver and skeletal muscle. Elevated levels of MyoD, reduced content of Sp1 and a changed ratio of Sp1 to Sp3 all together stimulate upregulation of PCTY2 transcription during C2C12 muscle cell differentiation. Disruption of the PCYT2 gene in mice leads to death after embryo implantation, establishing the necessity of PCYT2 for murine development.
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
CTP; phosphoethanolamine cytidylyltransferase; ET; Ethanolamine-phosphate cytidylyltransferase; Ethanolaminephosphate Cytidyltransferase; PCY2; PCY2_HUMAN; Pcyt2; Phosphate cytidylyltransferase 2 ethanolamine; Phosphorylethanolamine transferase.
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Also known as
PCYT2 Polyclonal Antibody
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Other name
Anti-PCYT2 Polyclonal
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
