Anti-NFIA/NF-1 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-11898R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  NFIA/NF-1
• Specificity
  This antibody reacts specifically with NFIA/NF-1
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human NFIA/NF-1
• Gene ID
  N/A
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, alpha-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  CCAAT binding transcription factor; CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; CTF; FLJ39164; NF I/A; NF-I/A; NF1 A; NF1-A; NF1A; NFI A; NFI L; NFI-A; NFIA; NFIA_HUMAN; Nuclear Factor 1 A; Nuclear factor 1 A type; Nuclear factor 1 A-type; Nuclear factor 1/A; Nuclear Factor 1A; Nuclear Factor I A; Nuclear factor I/A; nuclear factor I/X CCAAT-binding transcription factor; ynuclear factor I/X CCAAT-binding transcription factor; Nuclear factor I/X; Nuclear Factor IA; TGGCA binding protein; TGGCA-binding protein.
• Also known as
  NFIA/NF-1 Polyclonal Antibody
• Other name
  Anti-NFIA/NF-1 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  NFIA/NF-1  
• Gene symbol
  NF1, NFIA-AS2, NFIA, NFE4, NFE2, NFIA-AS1, GATA1, NEFH, YBX1, YY1
• Short name
  Anti-NFIA/NF-1
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  NFIA/NF-1 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:7765
• Gene
  NF1
• Long gene name
  neurofibromin 1
• Synonyms name
  • neurofibromatosis
  • von Recklinghausen disease
  • Watson disease
• Locus
  17q11.2
• Discovery year
  1986-01-01
• Entrez gene record
  4763
• Pubmed identfication
  • 1715669
• RefSeq identity
  • NM_000267
• Classification
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000132871
• Locus Specific Databases
  • Global Variome shared LOVD
  • LRG_214

Gene info

• Identity
  HGNC:40401
• Gene
  NFIA-AS2
• Long gene name
  NFIA antisense RNA 2
• Locus
  1p31.3
• Discovery year
  2014-05-21
• Entrez gene record
  100996570
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000008421

Gene info

• Identity
  HGNC:7784
• Gene
  NFIA
• Long gene name
  nuclear factor I A
• Synonyms gene name
  • nuclear factor I/A
• Synonyms
  • NFI-L
  • KIAA1439
• GenBank acession
  • U07809
• Locus
  1p31.3
• Discovery year
  1995-03-09
• Entrez gene record
  4774
• Pubmed identfication
  • 7590749
• RefSeq identity
  • NM_005595
• Classification
  • Nuclear factor I family
• VEGA ID
  OTTHUMG00000008618

Gene info

• Identity
  HGNC:29902
• Gene
  NFE4
• Long gene name
  nuclear factor, erythroid 4
• Synonyms
  • NF-E4
• Locus
  7q22.1
• Discovery year
  2013-08-23
• Entrez gene record
  58160
• Pubmed identfication
  • 11003662
• RefSeq identity
  • NR_166510
• VEGA ID
  OTTHUMG00000157208

Gene info

• Identity
  HGNC:7780
• Gene
  NFE2
• Long gene name
  nuclear factor, erythroid 2
• Synonyms gene name
  • nuclear factor (erythroid-derived 2), 45kD
  • nuclear factor (erythroid-derived 2), 45kDa
• Synonyms
  • NF-E2
• GenBank acession
  • BC005044
• Locus
  12q13.13
• Discovery year
  1993-11-03
• Entrez gene record
  4778
• Pubmed identfication
  • 8355703
• RefSeq identity
  • NM_006163
• Classification
  • Basic leucine zipper proteins
• VEGA ID
  OTTHUMG00000169761

Gene info

• Identity
  HGNC:40402
• Gene
  NFIA-AS1
• Long gene name
  NFIA antisense RNA 1
• Synonyms
  • RP5-833A20.1
• Locus
  1p31.3
• Discovery year
  2014-05-21
• Entrez gene record
  645030
• Pubmed identfication
  • 25265644
  • 27779670
• RefSeq identity
  • NR_104180
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000040631

Gene info

• Identity
  HGNC:4170
• Gene
  GATA1
• Long gene name
  GATA binding protein 1
• Synonyms gene
  • GF1
• Synonyms gene name
  • GATA-binding protein 1 (globin transcription factor 1)
  • globin transcription factor 1
• Synonyms
  • ERYF1
  • NFE1
  • GATA-1
  • NF-E1
• Synonyms name
  • nuclear factor, erythroid 1
• GenBank acession
  • X17254
• Locus
  Xp11.23
• Discovery year
  1990-09-10
• Entrez gene record
  2623
• Pubmed identfication
  • 1999341
• RefSeq identity
  • NM_002049
• Classification
  • GATA zinc finger domain containing
• VEGA ID
  OTTHUMG00000021504
• Locus Specific Databases
  • LRG_559

Gene info

• Identity
  HGNC:7737
• Gene
  NEFH
• Long gene name
  neurofilament heavy chain
• Synonyms gene name
  • neurofilament, heavy polypeptide 200kDa
  • neurofilament heavy polypeptide
  • neurofilament heavy
• Synonyms
  • NF-H
  • NFH
• Locus
  22q12.2
• Discovery year
  1988-08-31
• Entrez gene record
  4744
• RefSeq identity
  • NM_021076
• Classification
  • Intermediate filaments Type IV
• VEGA ID
  OTTHUMG00000151155
• Locus Specific Databases
  • ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

Gene info

• Identity
  HGNC:8014
• Gene
  YBX1
• Long gene name
  Y-box binding protein 1
• Synonyms gene
  • NSEP1
• Synonyms gene name
  • nuclease sensitive element binding protein 1
• Synonyms
  • YB-1
  • YB1
  • DBPB
  • NSEP-1
  • MDR-NF1
  • BP-8
  • CSDB
  • CSDA2
• GenBank acession
  • BC013838
• Locus
  1p34.2
• Discovery year
  1993-10-20
• Entrez gene record
  4904
• Pubmed identfication
  • 1891370
  • 3174636
  • 29478751
• RefSeq identity
  • NM_004559
• Classification
  • Y-box binding proteins
  • U11 small nuclear ribonucleoprotein
  • U11/U12 di-snRNP
• VEGA ID
  OTTHUMG00000007523

Gene info

• Identity
  HGNC:12856
• Gene
  YY1
• Long gene name
  YY1 transcription factor
• Synonyms
  • NF-E1
  • DELTA
  • UCRBP
  • YIN-YANG-1
  • INO80S
• Synonyms name
  • INO80 complex subunit S
  • Yin and Yang 1 protein
• GenBank acession
  • BC020324
• Locus
  14q32.2
• Discovery year
  1993-09-17
• Entrez gene record
  7528
• Pubmed identfication
  • 1655281
  • 7912122
• RefSeq identity
  • NM_003403
• Classification
  • INO80 complex
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000150479

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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