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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR® 594
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
MST4 + MST3 + STK25 Thr178 + Thr190 + Thr174
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Specificity
This antibody reacts specifically with MST4/MST3/STK25(T178/T190/T174)
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Modification
Phosphorylation
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Modification site
Thr178 + Thr190 + Thr174
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
N/A
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Antigen Source
KLH conjugated synthetic phosphopeptide derived from human MST-3 around the phosphorylation site of Thr178 + Thr190 + Thr174
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Gene ID
27347
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Swiss Prot
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
Novel human Ste20-related kinase Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation. It is pro apoptotic and is highly expressed in placenta, thymus, and peripheral blood leukocytes. Interaction with Golgi matrix protein GOLGA2 results in autophosphorylation on Thr-178, possibly as a consequence of stabilization of dimer formation. This may also be activated by C terminal cleavage. MST3 or Mammalian Sterile 20-like kinase 3 is a member of the germinal center kinase-III family. MST3 contains a conserved kinase domain at its NH(2)-terminus and a regulatory domain at its COOH-terminus. Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to nuclear translocation. Expression of COOH-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis. It can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through tyrosine phosphatase PTP-PEST. Mitogen activated protein kinase cascades have been conserved throughout evolution. In mammals, these cascades allow responses to complex stimuli such as growth factors and inflammatory cytokines. In yeast, STK25 functions upstream of the MAPK cascade.
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
MST4+MST3+STK25phospho T178 + T190 + T174;p-MST4 + MST3 + STK25 phospho T178 + T190 + T174;Mammalian STE20 like protein kinase 3; Mammalian Ste20 like protein kinase 4; Mammalian sterile 20 like 4; MASK; mess1; MGC94619; MST 3; MST 4; MST; Mst2; MST3 and SOK1 related kinase; MST3; MST3B; OTTHUMP00000018592; OTTHUMP00000018593; Protein kinase homolog serine/threonine kinase 3 Ste20 yeast homolog; RGD1563568; RP11-111L24.5; Serine/threonine kinase 24 STE20 homolog yeast; Serine/threonine kinase 24; Serine/threonine kinase 25; Serine/threonine protein kinase 24; Serine/threonine protein kinase MASK; Serine/threonine protein kinase MST 4; Serine/threonine protein kinase MST4; SOK1; STE20; Ste20 like; Ste20 like kinase; STE20 like kinase 3; STE20 like kinase MST 4; STE20 like kinase MST3; STE20 like kinase MST4; Ste20 yeast homolog; Ste20/oxidant stress response kinase 1; Sterile 20 oxidant stress response kinase 1; sterile 20 like kinase 3; Sterile 20/oxidant stress-response kinase 1; STK3; Yeast Sps1/Ste20 related kinase 1; YSK1.
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Also known as
MST4/MST3/STK25(T178/T190/T174) Antibody
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Other name
Anti-MST4/MST3/STK25(T178/T190/T174)
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
