-
Type
Conjugated Primary Antibody
-
Conjugated with
ALEXA FLUOR® 594
-
Host organism
Rabbit (Oryctolagus cuniculus)
-
Target Protein Peptide
LPCAT2
-
Specificity
This antibody reacts specifically with LPCAT2
-
Modification
No modification has been applied to this antibody
-
Modification site
None
-
Clonality
Polyclonal Antibody
-
Clone
Polyclonal Antibodies
-
Concentration
1ug per 1ul
-
Subcellular locations
Lumenal
-
Antigen Source
KLH conjugated synthetic peptide derived from human LPCAT2
-
Gene ID
54947
-
Swiss Prot
Q7L5N7
-
Applications
IF(IHC-P)
-
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
-
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
-
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
-
Background information
Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases. Also catalyzes the conversion of 1-acyl-sn-glycero-3-phosphocholine to 1,2-diacyl-sn-glycero-3-phosphocholine.
-
Purification method
Purified by Protein A.
-
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
-
Excitation emission
590nm/617nm
-
Synonyms
AYTL1; LysoPAFAT; Lysophosphatidylcholine acyltransferase 2; LPC acyltransferase 2; LPCAT-2; LysoPC acyltransferase 2; 1-acylglycerol-3-phosphate O-acyltransferase 11; 1-AGP acyltransferase 11; 1-AGPAT 11; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; Acyltransferase-like 1; Lysophosphatidic acid acyltransferase alpha; LPAAT-alpha; LPCAT2; AGPAT11
-
Also known as
LPCAT2 Polyclonal Antibody
-
Other name
Anti-LPCAT2 Polyclonal
-
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
-
Properties
For facs or microscopy Alexa 1 conjugate.
-
Conjugation
Alexa Fluor, ALEXA FLUOR® 594
-
Conjugated
Alexa conjugate 1
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
Gene target
-
Gene symbol
LPCAT2
-
Short name
Anti-LPCAT2
-
Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Isotype
Immunoglobulin G (IgG)
-
Label
ALEXA FLUOR 594
-
Alternative name
LPCAT2 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
-
Alternative technique
polyclonals, antibodies
-
Alternative to gene target
lysophosphatidylcholine acyltransferase 2, LPCAT2 and IDBG-31324 and ENSG00000087253 and 102725372,54947, transferase activity, Plasma membranes, Lpcat2 and IDBG-180294 and ENSMUSG00000033192 and 270084, LPCAT2 and IDBG-631933 and ENSBTAG00000019272 and 514646
-