Anti-Histone H2B (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-3652R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideHistone H2B
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SpecificityThis antibody reacts specifically with Histone H2B
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsNucleus
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Antigen SourceKLH conjugated synthetic peptide derived from human Histone H2B
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Gene ID3017. 8339. 8343. 8344. 8346. 8347.
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Swiss ProtN/A
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008].
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsH2B.1; H2B.1 B; H2B.b; H2B.c; H2B.d; H2B.e; H2B.f; H2B.j; H2B.q; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFJ; H2BFO; H2BFQ; H2BFS; HIRIP2; HIST1H2BB; HIST1H2BD; HIST1H2BH; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST2H2BE; Histone H2B; Histone H2B type 1 B; Histone H2B type 1 D; Histone H2B type 1 H; Histone H2B type 1 L; Histone H2B type 1 M; Histone H2B type 1 N; Histone H2B type 2 E; Histone protein; H2B GL105; H2B histone family member O; H2B histone family member S.
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Also known asHistone H2B Polyclonal Antibody
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Other nameAnti-Histone H2B Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolH2BC21, H2BC8, H2BC17, H2BC4, H2BC19P, H2BC20P, H2BC7, H2BC3, H2BC11, H2BC9
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Short nameAnti-Histone H2B
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameHistone H2B Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 21
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Synonyms gene
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Synonyms gene name
- H2B histone family, member Q
- histone 2, H2be
- histone cluster 2, H2be
- histone cluster 2 H2B family member e
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-08-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H2B histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 8
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Synonyms gene
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Synonyms gene name
- H2B histone family, member A
- histone 1, H2bg
- histone cluster 1, H2bg
- histone cluster 1 H2B family member g
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-08-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H2B histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 17
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Synonyms gene
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Synonyms gene name
- H2B histone family, member N
- histone 1, H2bo
- histone cluster 1, H2bo
- histone cluster 1 H2B family member o
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-08-27
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Entrez gene record
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Pubmed identfication
-
RefSeq identity
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Classification
- H2B histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 4
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Synonyms gene
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Synonyms gene name
- H2B histone family, member L
- histone 1, H2bc
- histone cluster 1, H2bc
- histone cluster 1 H2B family member c
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H2B histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 19, pseudogene
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Synonyms gene
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Synonyms gene name
- histone 2, H2bd
- H2B histone family, member O
- histone cluster 2, H2bd (pseudogene)
- histone cluster 2 H2B family member d (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-02-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H2B histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 20, pseudogene
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Synonyms gene
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Synonyms gene name
- histone 2, H2bc
- histone cluster 2, H2bc
- histone cluster 2, H2bc (pseudogene)
- histone cluster 2 H2B family member c (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-02-20
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Entrez gene record
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Pubmed identfication
-
RefSeq identity
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Classification
- H2B histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 7
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Synonyms gene
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Synonyms gene name
- H2B histone family, member G
- histone 1, H2bf
- histone cluster 1, H2bf
- histone cluster 1 H2B family member f
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-09-07
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Entrez gene record
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Pubmed identfication
-
RefSeq identity
-
Classification
- H2B histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 3
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Synonyms gene
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Synonyms gene name
- H2B histone family, member F
- histone 1, H2bb
- histone cluster 1, H2bb
- histone cluster 1 H2B family member b
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-09-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H2B histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 11
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Synonyms gene
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Synonyms gene name
- H2B histone family, member R
- histone 1, H2bj
- histone cluster 1, H2bj
- histone cluster 1 H2B family member j
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-10-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H2B histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 9
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Synonyms gene
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Synonyms gene name
- H2B histone family, member J
- histone 1, H2bh
- histone cluster 1, H2bh
- histone cluster 1 H2B family member h
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-08-27
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Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- H2B histones
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data