Anti-HCN2 + HCN4 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-11630R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  HCN2 + HCN4
• Specificity
  This antibody reacts specifically with HCN2 + HCN4
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human HCN2 + HCN4
• Gene ID
  N/A
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Hyperpolarization activated cation channels of the HCN gene family such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain. HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells; as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. HCN4 is another member of the family of hyperpolarization activated and cyclic nucleotide gated channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  BCNG 2; Brain cyclic nucleotide gated channel 2; HAC 1; HCN 4; HCN 2; Hyperpolarization activated cyclic nucleotide gated potassium channel 4;HCN2_HUMAN; HCN4_HUMAN.
• Also known as
  HCN2 + HCN4 Polyclonal Antibody
• Other name
  Anti-HCN2 + HCN4 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  HCN2   HCN4  
• Gene symbol
  HCN4, HCN2
• Short name
  Anti-HCN2 + HCN4
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  HCN2 + HCN4 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:16882
• Gene
  HCN4
• Long gene name
  hyperpolarization activated cyclic nucleotide gated potassium channel 4
• Synonyms gene name
  • hyperpolarization activated cyclic nucleotide-gated potassium channel 4
• GenBank acession
  • AJ132429
• Locus
  15q24.1
• Discovery year
  2002-09-02
• Entrez gene record
  10021
• Pubmed identfication
  • 10228147
  • 10430953
  • 16382102
• RefSeq identity
  • NM_005477
• Classification
  • Cyclic nucleotide gated channels
• VEGA ID
  OTTHUMG00000137563

Gene info

• Identity
  HGNC:4846
• Gene
  HCN2
• Long gene name
  hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2
• Synonyms gene
  • BCNG2
• Synonyms gene name
  • hyperpolarization activated cyclic nucleotide-gated potassium channel 2
  • hyperpolarization activated cyclic nucleotide gated potassium channel 2
• Synonyms
  • BCNG-2
  • HAC-1
• GenBank acession
  • AF064877
• Locus
  19p13.3
• Discovery year
  1998-08-20
• Entrez gene record
  610
• Pubmed identfication
  • 9405696
  • 9630217
  • 16382102
  • 10228147
  • 19584315
• RefSeq identity
  • NM_001194
• Classification
  • Cyclic nucleotide gated channels
• VEGA ID
  OTTHUMG00000180590

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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