Anti-ERK1(T202/Y204)+ERK2(T183/Y185), ALEXA Fluor 594

• Catalog number
  GENTObs-1646R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  ERK1 Thr202/Tyr204+ERK2 Thr183/Tyr185
• Specificity
  This antibody reacts specifically with ERK1(T202/Y204)+ERK2(T183/Y185)
• Modification
  Phosphorylation
• Modification site
  Thr183/Tyr185
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Cytoplasm
• Antigen Source
  KLH conjugated synthetic phosphopeptide derived from human ERK2 around the phosphorylation site of Thr183/Tyr185
• Gene ID
  5594,5595
• Swiss Prot
  P28482
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  ERK; p38; p4; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; Extracellular signal-regulated kinase 2; MAP kinase isoform p42; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1
• Also known as
  ERK1(T202/Y204)+ERK2(T183/Y185) Antibody
• Other name
  Anti-ERK1(T202/Y204)+ERK2(T183/Y185)
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.

• Gene target
  ERK1   T202/Y204   ERK2   T183/Y185  
• Gene symbol
  MAPK3, MAPK1
• Short name
  Anti-ERK1(T202/Y204)+ERK2(T183/Y185)
• Technique
  anti-, anti, antibody to, antibodies
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  ERK1(T202/Y204)+ERK2(T183/Y185) Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6877
• Gene
  MAPK3
• Long gene name
  mitogen-activated protein kinase 3
• Synonyms gene
  • PRKM3
• Synonyms
  • ERK1
  • p44mapk
  • p44erk1
• GenBank acession
  • M84490
• Locus
  16p11.2
• Discovery year
  1993-11-05
• Entrez gene record
  5595
• Pubmed identfication
  • 9628824
• Classification
  • Mitogen-activated protein kinases
• VEGA ID
  OTTHUMG00000132149

Gene info

• Identity
  HGNC:6871
• Gene
  MAPK1
• Long gene name
  mitogen-activated protein kinase 1
• Synonyms gene
  • PRKM2
  • PRKM1
• Synonyms
  • ERK
  • ERK2
  • p41mapk
  • MAPK2
• GenBank acession
  • M84489
• Locus
  22q11.22
• Discovery year
  1993-11-05
• Entrez gene record
  5594
• Classification
  • Mitogen-activated protein kinases
• VEGA ID
  OTTHUMG00000030508
• Locus Specific Databases
  • LRG_786

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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