Anti-EPHA2 (Polyclonal), ALEXA Fluor 350
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Catalog numberGENTObs-10209R-A350
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Price:
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 350
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideEPHA2
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SpecificityThis antibody reacts specifically with EPHA2 ALEXA FLUOR® 350
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsExtracellular
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Antigen SourceKLH conjugated synthetic peptide derived from human EPHA2
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Gene ID1969
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Swiss ProtP29317
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationReceptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission343nm/442nm
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SynonymsECK; CTPA; ARCC2; CTPP1; CTRCT6; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; EPHA2
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Also known asEPHA2 Polyclonal Antibody ALEXA FLUOR® 350
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Other nameAnti-EPHA2 Polyclonal ALEXA FLUOR® 350
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate. Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode.
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ConjugationAlexa Fluor, ALEXA FLUOR 350
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolEPHA2, EPHA2-AS1
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Short nameAnti-EPHA2 ALEXA FLUOR® 350
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 350
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Alternative nameEPHA2 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
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Alternative techniquepolyclonals, antibodies
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Alternative to gene targetEPH receptor A2, ARCC2 and CTPA and CTPP1 and CTRCT6 and ECK, EPHA2 and IDBG-91407 and ENSG00000142627 and 1969, transferase activity, Cell surfaces, Epha2 and IDBG-202005 and ENSMUSG00000006445 and 13836, EPHA2 and IDBG-645870 and ENSBTAG00000000815 and 512798
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Gene info
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Identity
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Gene
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Long gene nameEPH receptor A2
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Synonyms gene
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Synonyms gene name
- EphA2
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GenBank acession
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Locus
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Discovery year1991-08-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Sterile alpha motif domain containing
- EPH receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameEPHA2 antisense RNA 1
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Locus
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Discovery year2021-07-29
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Entrez gene record
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Classification
- Antisense RNAs
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data