Anti-eIF3K (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-12057R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein Peptidee3K
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SpecificityThis antibody reacts specifically with eIF3K
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsN/A
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Antigen SourceKLH conjugated synthetic peptide derived from human eIF3K
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Gene IDN/A
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Swiss ProtN/A
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationeIF3K (Eukaryotic translation initiation factor 3 subunit K, Muscle-specific gene M9 protein) is a widely expressed translation initiation factor that belongs to the eIF3 subunit K family. Translation initiation factor 3 (eIF3) is a multisubunit complex containing at least 12 subunits. eIF3 binds to the 40S ribosomal subunit, promotes the binding of methionyl-tRNAi and mRNA, and interacts with several other initiation factors to form the 40S initiation complex. eIF3K is the smallest subunit of eIF3 and it interacts with several other subunits of eIF3 and the 40S ribosomal subunit. eIF3K is conserved among high eukaryotes, including mammals, insects, and plants, and it is ubiquitously expressed in human tissues. eIF3K is distributed both in nucleus and cytoplasm and colocalizes with cyclin D3, a regulatory subunit of cyclin-dependent kinase 4 (Cdk4).
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsARG134; e 3 p25; e 3 p28; e-3 p25; e-3 p28; E3-p28; e3k; e3k; E3K_HUMAN; E3S12; Eukaryotic translation initiation factor 3 subunit 12; Eukaryotic translation initiation factor 3 subunit K; HSPC029; M9; MSTP001; Muscle specic gene M9 protein; Muscle-specic gene M9 protein; PLAC 24; PLAC-24; PLAC24; PRO1474; PTD001; E3K_HUMAN.
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Also known aseIF3K Polyclonal Antibody
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Other nameAnti-eIF3K Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolEIF3K
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Short nameAnti-eIF3K
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameeIF3K Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameeukaryotic translation initiation factor 3 subunit K
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Synonyms gene
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Synonyms gene name
- eukaryotic translation initiation factor 3, subunit 12
- eukaryotic translation initiation factor 3, subunit K
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-01-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Eukaryotic translation initiation factor 3
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data