Anti-DIP13B/APPL2 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-13631R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  DIP13B/APPL2
• Specificity
  This antibody reacts specifically with DIP13B/APPL2
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human DIP13B/APPL2
• Gene ID
  N/A
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  The APPL family of proteins are involved in linking, trafficking and signaling downstream of tyrosine kinase receptors. APPL1, also designated adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; APPL; or DCC interacting protein 13å (DIP13å), and APPL2, also designated adaptor protein containing pH domain, PTB domain and leucine zipper motif 2 or DCC interacting protein 13∫ (DIP13∫), are involved in the coupling of epidermal growth factor (EGF) signaling and chromatin remodeling in the nucleus. They associate with GTPase Rab 5 and are released from the plasma membrane and translocated to the nucleus. In the nucleus, APPL1 and APPL2 associate with NuRD/MeCP1 and are essential for cell growth and proliferation. APPL2 also associates with follicle stimulating hormone receptor (FSHR). APPL2 is highly expressed in heart, brain, skeletal muscle, and kidney. APPL2 shares 54% homology with APPL1
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  Adapter protein containing PH domain; Adapter protein containing PH domain PTB domain and leucine zipper mot 2; adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2; APLP2; Appl2; DCC interacting protein 13 beta; DCC-interacting protein 13-beta; Dip13 beta; Dip13-beta; DP13B_HUMAN; PTB domain and leucine zipper mot 2.
• Also known as
  DIP13B/APPL2 Polyclonal Antibody
• Other name
  Anti-DIP13B/APPL2 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  DIP13B/APPL2  
• Gene symbol
  APPL2, APLP2
• Short name
  Anti-DIP13B/APPL2
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  DIP13B/APPL2 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:18242
• Gene
  APPL2
• Long gene name
  adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2
• Synonyms gene name
  • adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
• Synonyms
  • FLJ10659
  • DIP13B
• Synonyms name
  • DCC-interacting protein 13-beta
• GenBank acession
  • AY113704
• Locus
  12q23.3
• Discovery year
  2007-01-26
• Entrez gene record
  55198
• Pubmed identfication
  • 11431708
  • 17030088
• RefSeq identity
  • NM_018171
• Classification
  • BAR-PH domain containing
• VEGA ID
  OTTHUMG00000169853

Gene info

• Identity
  HGNC:598
• Gene
  APLP2
• Long gene name
  amyloid beta precursor like protein 2
• Synonyms gene
  • APPL2
• Synonyms gene name
  • amyloid beta (A4) precursor-like protein 2
• Synonyms
  • APPH
• GenBank acession
  • L19597
• Locus
  11q24.3
• Discovery year
  1993-06-23
• Entrez gene record
  334
• Pubmed identfication
  • 10702673
• RefSeq identity
  • NM_001642
• VEGA ID
  OTTHUMG00000165767

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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