Anti-DARS2 (Polyclonal), Biotin
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Catalog numberGENTObs-14198R-Biotin
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Price:
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withBiotin
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideDARS2
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SpecificityThis antibody reacts specifically with DARS2 Biotin
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsNucleus, Mitochondrion
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Antigen SourceKLH conjugated synthetic peptide derived from human DARS2
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Gene ID55157
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Swiss ProtQ6PI48
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ApplicationsIHC-P
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Applications with corresponding dilutionsIHC-P(1:100-500)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emissionN/A
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SynonymsLBSL; ASPRS; MT-ASPRS; Aspartate--tRNA ligase, mitochondrial; Aspartyl-tRNA synthetase; DARS2
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Also known asDARS2 Polyclonal Antibody Biotin
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Other nameAnti-DARS2 Polyclonal Biotin
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolDARS2
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Short nameAnti-DARS2 Biotin
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelBiotin
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Alternative nameDARS2 Polyclonal Antibody, Biotin Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameaspartyl-tRNA synthetase 2, mitochondrial
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-06-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Aminoacyl tRNA synthetases, Class II
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data