Anti-CSTF3/CSTF77 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-12935R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  CSTF3/CSTF77
• Specificity
  This antibody reacts specifically with CSTF3/CSTF77
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human CSTF3/CSTF77
• Gene ID
  1479
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA. CstF-77 (cleavage stimulation factor, 77 kDa subunit), also known as CstF3, is one of the three subunits comprising CstF. It can exist as a homodimer and functions as the bridge, directly interacting with the other two CstF subunits, namely CstF-64 and CstF-50. CstF-77 is highly conserved among eukaryotes. It contains an Alpha-helical structure with 11 HAT (Half-a-TPR-containing) repeats and is essential for CstF assembly. In addition, CstF-77 is capable of interacting with CPSF1 and FCP1, other factors involved in polyadenylation.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  CF 1 77 kDa subunit; Cleavage stimulation factor 77 kDa subunit; Cleavage stimulation factor subunit 3; Cleavage stimulation factor subunit 3 isoform 1; CSTF 3; CSTF 77; CSTF 77 kDa subunit; CSTF77; MGC117398; MGC43001; MGC75122; CSTF3_HUMAN.
• Also known as
  CSTF3/CSTF77 Polyclonal Antibody
• Other name
  Anti-CSTF3/CSTF77 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  CSTF3/CSTF77  
• Gene symbol
  CSTF3-DT, CSTF3
• Short name
  Anti-CSTF3/CSTF77
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  CSTF3/CSTF77 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:27882
• Gene
  CSTF3-DT
• Long gene name
  CSTF3 divergent transcript
• Synonyms gene
  • CSTF3-AS1
• Synonyms gene name
  • CSTF3 antisense RNA 1 (head to head)
• Locus
  11p13
• Discovery year
  2013-01-23
• Entrez gene record
  338739
• Pubmed identfication
  • 12477932
• RefSeq identity
  • NR_034027
• Classification
  • Divergent transcripts
• VEGA ID
  OTTHUMG00000166243

Gene info

• Identity
  HGNC:2485
• Gene
  CSTF3
• Long gene name
  cleavage stimulation factor subunit 3
• Synonyms gene name
  • cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kD
  • cleavage stimulation factor, 3' pre-RNA, subunit 3
• Synonyms
  • CstF-77
• GenBank acession
  • U15782
• Locus
  11p13
• Discovery year
  1995-01-16
• Entrez gene record
  1479
• Pubmed identfication
  • 7984242
• RefSeq identity
  • NM_001326
• Classification
  • Cleavage stimulation factor subunits
• VEGA ID
  OTTHUMG00000166268

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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