Anti-CSP (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-12944R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideCSP
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SpecificityThis antibody reacts specifically with CSP
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsN/A
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Antigen SourceKLH conjugated synthetic peptide derived from human CSP
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Gene ID80331
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Swiss ProtN/A
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationCysteine string proteins (CSPs) are synaptic vesicle-associated, secretory vesicle proteins that are involved in Ca2+-regulated exocytosis of synaptic vesicles and modulation of presynaptic transmembrane calcium fluxes in neuroendocrine and endocrine cell types. CSP contains a J-domain that binds HSP 70/HSC 70 chaperone ATPases and a membrane-targeting, palmitoylated cysteine-rich string region. CSPs may act as molecular chaperones in synapses, and mediate conformational folding of components of the vesicular exocytotic machinery. CSP is involved in the fine tuning of neurotransmission through its interaction with receptor-coupled trimeric GTP binding proteins (G proteins) and N-type Ca2+ channels. Two variants of CSP have been described: CSP1; and the 31 amino acid, C-terminally truncated isoform, CSP2. Subcellular fractionation of insulinoma cells shows CSP1 in granular fractions, while the membrane and cytosol fractions contain predominantly CSP2. The fractions also contain additional proteins, presumably CSP dimers. Furthermore, in various mammalian cell lines (including rat brain) CSP1 expression predominates CSP2 expression.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsCLN 4; CLN4; CLN4B; CSP; cysteine string protein alpha; Cysteine string protein; DJC5; DnaJ Hsp40 homolog subfamily C member 5; DnaJ homolog subfamily C member 5; DNAJC 5; Dnajc5; DNAJC5A; DNJC5_HUMAN; NCL.
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Also known asCSP Polyclonal Antibody
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Other nameAnti-CSP Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolDNAJC5G, DNAJC5B, GZMH, GZMB
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Short nameAnti-CSP
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameCSP Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameDnaJ heat shock protein family (Hsp40) member C5 gamma
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Synonyms gene name
- DnaJ (Hsp40) homolog, subfamily C, member 5 gamma
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-03-16
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Entrez gene record
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RefSeq identity
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Classification
- DNAJ (HSP40) heat shock proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameDnaJ heat shock protein family (Hsp40) member C5 beta
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Synonyms gene name
- DnaJ (Hsp40) homolog, subfamily C, member 5 beta
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-03-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DNAJ (HSP40) heat shock proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegranzyme H
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Synonyms gene
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Synonyms gene name
- granzyme H (cathepsin G-like 2, protein h-CCPX)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1990-04-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Granule associated serine proteases of immune defence
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegranzyme B
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Synonyms gene
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Synonyms gene name
- granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-05-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Granule associated serine proteases of immune defence
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data