Anti-Clathrin heavy chain (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-2932R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  Clathrin heavy chain
• Specificity
  This antibody reacts specifically with Clathrin heavy chain
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Cytoplasm
• Antigen Source
  KLH conjugated synthetic peptide derived from human Clathrin heavy chain.
• Gene ID
  1213, 8218
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008].
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  CHC17; Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain; Clathrin heavy chain like 1; Clathrin heavy chain on chromosome 17; Clathrin heavy polypeptide; CLH 17; CLH 22; CLH-17; CLH1_HUMAN; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034.
• Also known as
  Clathrin heavy chain Polyclonal Antibody
• Other name
  Anti-Clathrin heavy chain Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  Clathrin   heavy   chain  
• Gene symbol
  CLTC, CLTCL1, CLHC1
• Short name
  Anti-Clathrin heavy chain
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  Clathrin heavy chain Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:2092
• Gene
  CLTC
• Long gene name
  clathrin heavy chain
• Synonyms gene
  • CLTCL2
• Synonyms gene name
  • clathrin, heavy polypeptide (Hc)
  • clathrin, heavy chain
  • clathrin, heavy polypeptide-like 2
  • clathrin, heavy chain (Hc)
• Synonyms
  • Hc
• GenBank acession
  • X55878
• Locus
  17q23.1
• Discovery year
  1990-10-16
• Entrez gene record
  1213
• Pubmed identfication
  • 1765375
  • 7584026
• RefSeq identity
  • NM_004859
• Classification
  • Clathrin subunits
• VEGA ID
  OTTHUMG00000134279

Gene info

• Identity
  HGNC:2093
• Gene
  CLTCL1
• Long gene name
  clathrin heavy chain like 1
• Synonyms gene
  • CLTCL
• Synonyms gene name
  • clathrin, heavy polypeptide-like 1
  • clathrin, heavy chain-like 1
• Synonyms
  • CLTD
  • CLH22
  • CHC22
• Locus
  22q11.21
• Discovery year
  1995-09-08
• Entrez gene record
  8218
• Pubmed identfication
  • 8844170
  • 15133132
• RefSeq identity
  • NM_007098
• Classification
  • Clathrin subunits
• VEGA ID
  OTTHUMG00000150109

Gene info

• Identity
  HGNC:26453
• Gene
  CLHC1
• Long gene name
  clathrin heavy chain linker domain containing 1
• Synonyms gene
  • C2orf63
• Synonyms gene name
  • chromosome 2 open reading frame 63
• Synonyms
  • FLJ31438
• Locus
  2p16.1
• Discovery year
  2007-12-07
• Entrez gene record
  130162
• RefSeq identity
  • NM_152385
• VEGA ID
  OTTHUMG00000151918

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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