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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR® 594
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
CHPT1
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Specificity
This antibody reacts specifically with CHPT1
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Modification
No modification has been applied to this antibody
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Modification site
None
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
N/A
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Antigen Source
KLH conjugated synthetic peptide derived from human CHPT1
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Gene ID
56994
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Swiss Prot
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
CHPT1, also known as AAPT1-like protein and Diacylglycerol cholinephosphotransferase 1, is a 406 amino acid multi-pass membrane protein that is localized to the golgi apparatus. By catalyzing the phosphatidylcholine biosynthesis from CDP-choline, it plays an essential role in the formation and maintenance of vesicular membranes. CHPT1 is most abundant in testis, as well as small intestine, heart, colon, spleen and prostate. Expression of CHPT1 is increased in cancerous breast cells as compared to normal breast cell lines and it has been determined that the CHPT1 gene exhibits mutations within the cancerous cells. Interestingly, exposure to mustard gas significantly decreases CHPT1 gene expression and activity, an event that may play an important role in the development of acute respiratory distress syndrome (ARDS). There are two isoforms of CHPT1 that are produced as a result of alternative splicing events.
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
AAPT1-like protein; Cholinephosphotransferase 1; chpt1; CHPT1_HUMAN; Diacylglycerol cholinephosphotransferase 1; hCPT1.
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Also known as
CHPT1 Polyclonal Antibody
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Other name
Anti-CHPT1 Polyclonal
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbol
CHPT1
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Short name
Anti-CHPT1
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Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 594
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Alternative name
CHPT1 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
choline phosphotransferase 1, CHPT1 and IDBG-53342 and ENSG00000111666 and 56994, phosphotransferase activity, Plasma membranes, Chpt1 and IDBG-183566 and ENSMUSG00000060002 and 212862, CHPT1 and IDBG-637087 and ENSBTAG00000002490 and 511291
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