Anti-AIF (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-0037R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  AIF
• Specificity
  This antibody reacts specifically with AIF
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Cytoplasm, Mitochondrion
• Antigen Source
  KLH conjugated synthetic peptide derived from human AIF
• Gene ID
  9131
• Swiss Prot
  O95831
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus), Pig/Swine (Sus scrofa)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6; Apoptosis-inducing factor 1, mitochondrial; Programmed cell death protein 8; AIFM1
• Also known as
  AIF Polyclonal Antibody
• Other name
  Anti-AIF Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  AIF  
• Gene symbol
  AIFM1, AIF1
• Short name
  Anti-AIF
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  AIF Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:8768
• Gene
  AIFM1
• Long gene name
  apoptosis inducing factor mitochondria associated 1
• Synonyms gene
  • PDCD8
  • NAMSD
  • AUNX1
• Synonyms gene name
  • programmed cell death 8 (apoptosis-inducing factor)
  • neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome)
  • apoptosis-inducing factor, mitochondrion-associated, 1
  • apoptosis inducing factor, mitochondria associated 1
  • auditory neuropathy, X-linked recessive 1
• Synonyms
  • AIF
  • CMTX4
  • DFNX5
• GenBank acession
  • AF100928
• Locus
  Xq26.1
• Discovery year
  1999-05-28
• Entrez gene record
  9131
• Pubmed identfication
  • 9989411
  • 23217327
  • 25986071
• VEGA ID
  OTTHUMG00000022392
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:352
• Gene
  AIF1
• Long gene name
  allograft inflammatory factor 1
• Synonyms
  • IRT-1
  • AIF-1
  • Em:AF129756.17
  • IBA1
• Synonyms name
  • interferon gamma responsive transcript
  • ionized calcium-binding adapter molecule 1
• GenBank acession
  • U19713
• Locus
  6p21.33
• Discovery year
  1997-07-01
• Entrez gene record
  199
• Pubmed identfication
  • 8912632
• Classification
  • EF-hand domain containing
• VEGA ID
  OTTHUMG00000031246

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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