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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR 594
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Host organism
Mouse (Mus musculus)
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Target Protein Peptide
P53wt-p53
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Specificity
Highly specific antibody against P53wt-p53
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Modification
Not modified
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Clonality
Monoclonal Antibodies
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Clone
D2F7
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Concentration
1ug per 1ul
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Antigen Source
KLH conjugated synthetic peptide derived from human P53(wt-p53)
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Gene ID
7157
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross Reactive species
Human (Homo sapiens), Rat (Rattus norvegicus)
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Cross Reactive Species details
No significant cross reactivity is observed
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Background information
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013].
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Purification method
Purified by Protein G.
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Storage
Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
Widespread p53; Wtp53; Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; HGNC11998; LFS1; p53; p53 Cellular Tumor Antigen; p53 Tumor Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumor protein p53; Tumour Protein p53; P53_HUMAN; TP53.
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Alternative name 1
P53(wt-p53) (D2F7) Monoclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative name 2
P53(wt-p53) (D2F7) Monoclonal anti-, ALEXA FLUOR 594 Conjugated
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Advisory
Cycles of freezing and thawing should be avoided as such cycles may denature the peptide chains of the antibody and reduce its affinity, specificity and reactivity. For antibodies in a liquid form or reconstituted lyophilized antibodies - small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
